TY - JOUR
T1 - Expression of the Bacillus thuringiensis mosquitocidal toxin Cry11Aa in the aquatic bacterium Asticcacaulis excentricus
AU - Armengol, Gemma
AU - Guevara, Oscar Enrique
AU - Orduz, Sergio
AU - Crickmore, Neil
PY - 2005/12/1
Y1 - 2005/12/1
N2 - A mosquitocidal aquatic bacterium has been developed by introducing an operon containing the cry11Aa, and p20 genes from Bacillus thuringiensis subsp. israelensis (Bti) into the gram-negative aquatic bacterium Asticcacaulis excentricus. After transformation, the cry11Aa gene was successfully expressed in recombinant A. excentricus under the tac promoter, at the level of 0.04 pg/cell. The recombinant bacteria were toxic to Aedes aegypti larvae with an LC50 of 6.83 × 105 cells/mL. We believe that these bacteria may have potential as genetically engineered microorganisms for the control of mosquito larvae. © Springer Science+Business Media, Inc. 2005.
AB - A mosquitocidal aquatic bacterium has been developed by introducing an operon containing the cry11Aa, and p20 genes from Bacillus thuringiensis subsp. israelensis (Bti) into the gram-negative aquatic bacterium Asticcacaulis excentricus. After transformation, the cry11Aa gene was successfully expressed in recombinant A. excentricus under the tac promoter, at the level of 0.04 pg/cell. The recombinant bacteria were toxic to Aedes aegypti larvae with an LC50 of 6.83 × 105 cells/mL. We believe that these bacteria may have potential as genetically engineered microorganisms for the control of mosquito larvae. © Springer Science+Business Media, Inc. 2005.
U2 - 10.1007/s00284-005-0166-z
DO - 10.1007/s00284-005-0166-z
M3 - Article
SN - 0343-8651
VL - 51
SP - 430
EP - 433
JO - Current Microbiology
JF - Current Microbiology
ER -