@article{2cc2f00c0bd645b898fe47cb63aa55b2,
title = "Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos",
abstract = "This study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1, produced by Antarctic Pseudomonas sp., in the vitrification of in vitro-produced (IVP) bovine embryos. IVP day 7 (D7) and day 8 (D8) expanded blastocysts derived from cow or calf oocytes were vitrified without supplementation (EPS0) or supplemented with 10 mu g/mL (EPS10) or 100 mu g/mL (EPS100) EPS ID1. The effect of EPS ID1 was assessed in post-warming re-expansion and hatching rates, differential cell count, apoptosis rate, and gene expression. EPS100 re-expansion rates were significantly higher than those observed for the EPS0 and EPS10 treatments, regardless of culture length or oocyte source. EPS100 hatching rate was similar to the one of the fresh blastocysts except for those D7 blastocysts derived from calf oocytes. No differences were observed among EPS ID1 treatments when the inner cell mass, trophectoderm, and total cell number were assessed. Although apoptosis rates were higher (p",
keywords = "TUNEL, blastocyst, cryopreservation, embryo development, gene expression regulation, inner cell mass, total cell number, Vitrification, Blastocyst, Fertilization in Vitro/methods, Cryopreservation/methods, bcl-2-Associated X Protein/genetics, Animals, Cattle, Embryo Culture Techniques/methods, Female, SURVIVAL, HYPOTHERMIC STORAGE, IN-VITRO, PROTEIN TYPE-III, BLASTOCYSTS, ANTIFREEZE PROTEINS, MATURE MOUSE OOCYTES, CULTURE, BACTERIUM, EXPRESSION",
author = "Ord{\'o}{\~n}ez-Le{\'o}n, {Erika Alina} and Iris Mart{\'i}nez-Rodero and Tania Garc{\'i}a-Mart{\'i}nez and Manel L{\'o}pez-B{\'e}jar and Marc Yeste and Elena Mercade and Teresa Mogas",
note = "Funding Information: This study was supported by the Spanish Ministry of Science and Innovation (Project PID2020-116531RB-I00) and Generalitat de Catalunya (Project No. 2017 SGR 1229). Alina Ord{\'o}{\~n}ez-Le{\'o}n was awarded a post-doctoral scholarship from the Mexican National Conseil for Science and Technology (CONACYT-45193). Iris Mart{\'i}nez-Rodero was awarded a scholarship from the Spanish Ministry of Science, Innovation, and Universities (BES-2017-081962). Funding Information: Funding: This study was supported by the Spanish Ministry of Science and Innovation (Project PID2020-116531RB-I00) and Generalitat de Catalunya (Project No. 2017 SGR 1229). Alina Ord{\'o}{\~n}ez-Le{\'o}n was awarded a post-doctoral scholarship from the Mexican National Conseil for Science and Technology (CONACYT-45193). Iris Mart{\'i}nez-Rodero was awarded a scholarship from the Spanish Ministry of Science, Innovation, and Universities (BES-2017-081962). Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2022",
month = jul,
doi = "10.3390/ijms23137069",
language = "English",
volume = "23",
journal = "International journal of molecular sciences",
issn = "1661-6596",
number = "13",
}