Enzymatic determination of anti-NAD+ glycohydrolase in serum

F. J. Gella; J. Viver; F. Canalias; J. Beleta; F. Gonzalez-Sastre

Enzymatic determination of anti-NAD⁺ glycohydrolase in serum

F. J. Gella, J. Viver, F. Canalias, J. Beleta, F. Gonzalez-Sastre

Departament de Bioquímica i de Biologia Molecular

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Resum

S'ha desenvolupat un mètode colorimètric per mesurar la glicohidrolasa anti-NAD+ en el sèrum humà. L'assaig implica la inhibició de la glicohidrolasa anti-NAD+ (EC 3.2.2.5) mitjançant l'anticòs i la determinació de l'activitat enzimàtica no inhibida utilitzant un sistema d'amplificació enzimàtic per NAD+. L'assaig es porta a terme fàcilment i té l'avantatge addicional d'una relació directa entre el senyal i la concentració d'anticossos. Els resultats obtinguts en 100 sèrums humans es comparen favorablement amb altres proves que s'utilitzen habitualment per obtenir evidència d'infeccions estreptocòcciques o les seves complicacions, com ara els tests anti-estreptolisina O i anti-DNasa B.

Idioma original	Anglès
Pàgines (de-a)	1007-1011
Revista	Clin. Chem.
Volum	39
Número	6
Estat de la publicació	Publicada - 1 de gen. 1993

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@article{52c80fb686214bb89f4b8355becba747,

title = "Enzymatic determination of anti-NAD+ glycohydrolase in serum",

abstract = "A colorimetric method for measuring anti-NAD+ glycohydrolase in human sera has been developed. The assay involves the inhibition of NAD+ glycohydrolase (EC 3.2.2.5) by the antibody and determination of the noninhibited enzyme activity by using an enzymatic amplifying system for NAD+. The assay is easily carried out and has the additional advantage of a direct relationship between signal and antibody concentration. The results obtained for 100 human sera compare favorably with other tests commonly used to obtain evidence of streptococcal infections or their complications, such as the anti-streptolysin O and the anti-DNase B tests.",

keywords = "bacterial toxins, DNase B, enzyme inhibition assay, infection, streptolysin O",

author = "Gella, \{F. J.\} and J. Viver and F. Canalias and J. Beleta and F. Gonzalez-Sastre",

year = "1993",

month = jan,

day = "1",

language = "English",

volume = "39",

pages = "1007--1011",

journal = "Clin. Chem.",

issn = "0009-9147",

publisher = "Oxford University Press",

number = "6",

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TY - JOUR

T1 - Enzymatic determination of anti-NAD+ glycohydrolase in serum

AU - Gella, F. J.

AU - Viver, J.

AU - Canalias, F.

AU - Beleta, J.

AU - Gonzalez-Sastre, F.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - A colorimetric method for measuring anti-NAD+ glycohydrolase in human sera has been developed. The assay involves the inhibition of NAD+ glycohydrolase (EC 3.2.2.5) by the antibody and determination of the noninhibited enzyme activity by using an enzymatic amplifying system for NAD+. The assay is easily carried out and has the additional advantage of a direct relationship between signal and antibody concentration. The results obtained for 100 human sera compare favorably with other tests commonly used to obtain evidence of streptococcal infections or their complications, such as the anti-streptolysin O and the anti-DNase B tests.

AB - A colorimetric method for measuring anti-NAD+ glycohydrolase in human sera has been developed. The assay involves the inhibition of NAD+ glycohydrolase (EC 3.2.2.5) by the antibody and determination of the noninhibited enzyme activity by using an enzymatic amplifying system for NAD+. The assay is easily carried out and has the additional advantage of a direct relationship between signal and antibody concentration. The results obtained for 100 human sera compare favorably with other tests commonly used to obtain evidence of streptococcal infections or their complications, such as the anti-streptolysin O and the anti-DNase B tests.

KW - bacterial toxins

KW - DNase B

KW - enzyme inhibition assay

KW - infection

KW - streptolysin O

UR - https://www.scopus.com/pages/publications/0027232036

M3 - Article

SN - 0009-9147

VL - 39

SP - 1007

EP - 1011

JO - Clin. Chem.

JF - Clin. Chem.

IS - 6

ER -

Enzymatic determination of anti-NAD+ glycohydrolase in serum

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Enzymatic determination of anti-NAD⁺ glycohydrolase in serum