TY - JOUR
T1 - Electrochromic biosensors based on screen-printed Prussian Blue electrodes
AU - Aller-Pellitero, Miguel
AU - Fremeau, Joséphine
AU - Villa, Rosa
AU - Guirado, Gonzalo
AU - Lakard, Boris
AU - Hihn, Jean Yves
AU - del Campo, F. Javier
PY - 2019/7/1
Y1 - 2019/7/1
N2 - © 2019 Elsevier B.V. Prussian Blue (PB)-modified graphite screen-printed electrodes are increasingly being used in electrochemical biosensors. However, they do not allow the observation of the electrochromism of PB. This work presents the construction of PB-based, electrochromic screen-printed biosensors. Although electrically more resistive than their graphite counterparts, these new PB-based electrodes enable both the amperometric and colorimetric detection of hydrogen peroxide. This is the first time that this has been achieved using screen-printed electrodes, and we demonstrate it spectroelectrochemically on a glucose biosensor. The biosensor electrochemical performance equals that of previously reported PB/graphite electrodes, being able to detect down to 4 μM H2O2 and 54 μM glucose. At the same time, and in contrast to PB/graphite electrodes, the new PB-based electrodes afford the optical detection of these two analytes down to 1.2 μM and 15 μM, respectively. The dynamic ranges of the glucose biosensors obtained at the PB-based electrodes are 0.1–1 mM (amperometric) and 0.025–2.5 mM (Colorimetric), matching the physiological glucose concentration range in body fluids other than blood or serum.
AB - © 2019 Elsevier B.V. Prussian Blue (PB)-modified graphite screen-printed electrodes are increasingly being used in electrochemical biosensors. However, they do not allow the observation of the electrochromism of PB. This work presents the construction of PB-based, electrochromic screen-printed biosensors. Although electrically more resistive than their graphite counterparts, these new PB-based electrodes enable both the amperometric and colorimetric detection of hydrogen peroxide. This is the first time that this has been achieved using screen-printed electrodes, and we demonstrate it spectroelectrochemically on a glucose biosensor. The biosensor electrochemical performance equals that of previously reported PB/graphite electrodes, being able to detect down to 4 μM H2O2 and 54 μM glucose. At the same time, and in contrast to PB/graphite electrodes, the new PB-based electrodes afford the optical detection of these two analytes down to 1.2 μM and 15 μM, respectively. The dynamic ranges of the glucose biosensors obtained at the PB-based electrodes are 0.1–1 mM (amperometric) and 0.025–2.5 mM (Colorimetric), matching the physiological glucose concentration range in body fluids other than blood or serum.
KW - Biosensors
KW - Glucose sensing
KW - Hydrogen peroxide detection
KW - Prussian Blue electrodes
KW - Screen-printed electrodes
KW - Spectroelectrochemistry
U2 - 10.1016/j.snb.2019.03.100
DO - 10.1016/j.snb.2019.03.100
M3 - Article
SN - 0925-4005
VL - 290
SP - 591
EP - 597
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -