TY - JOUR
T1 - Electrochemical detection in vitro and electron transfer mechanism of testosterone using a modified electrode with a cobalt oxide film
AU - Moura, Silio Lima
AU - Moraes, Rafael Rodrigues De
AU - Santos, Marcos Antônio Pereira Dos
AU - Moreira, Davyson De Lima
AU - Lopes, José Arimatéia Dantas
AU - Pividori, Maria Isabel
AU - Zucolotto, Valtencir
AU - Júnior, José Ribeiro Dos Santos
PY - 2014/10/31
Y1 - 2014/10/31
N2 - The voltammetric oxidation of testosterone (4-androsten-17β-ol-3-one) has been studied at an edge plane glassy carbon electrode modified with cobalt oxide. One sensitive and well-defined peak is obtained with a peak potential of 0.50 V vs. SCE attributed to the reduction reaction CoIV/Co III, which is responsible for oxidation of the steroid. A calibration curve was obtained for testosterone, with the measurements performed in triplicate, in the range from 0.33 to 2.00 μM, yielding a correlation coefficient >0.99 and detection limit of 0.16 μM, with relative standard deviations (R.S.D) from 1.33 × 10-5 to 4.00 × 10 -4% with 95% significance (p < 0.05). The modified electrode showed a stable and reproducible response toward the detection of this steroid. Thus, the results showed that the electrode modified with cobalt oxide acts as a promising alternative for simple, cheap and rapid analysis of testosterone. © 2014 Elsevier B.V.
AB - The voltammetric oxidation of testosterone (4-androsten-17β-ol-3-one) has been studied at an edge plane glassy carbon electrode modified with cobalt oxide. One sensitive and well-defined peak is obtained with a peak potential of 0.50 V vs. SCE attributed to the reduction reaction CoIV/Co III, which is responsible for oxidation of the steroid. A calibration curve was obtained for testosterone, with the measurements performed in triplicate, in the range from 0.33 to 2.00 μM, yielding a correlation coefficient >0.99 and detection limit of 0.16 μM, with relative standard deviations (R.S.D) from 1.33 × 10-5 to 4.00 × 10 -4% with 95% significance (p < 0.05). The modified electrode showed a stable and reproducible response toward the detection of this steroid. Thus, the results showed that the electrode modified with cobalt oxide acts as a promising alternative for simple, cheap and rapid analysis of testosterone. © 2014 Elsevier B.V.
KW - Antidoping
KW - Cobalt oxide
KW - Electrochemical sensor
KW - Electron transfer
KW - Testosterone
U2 - 10.1016/j.snb.2014.05.104
DO - 10.1016/j.snb.2014.05.104
M3 - Article
SN - 0925-4005
VL - 202
SP - 469
EP - 474
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -