DNA vaccination techniques

Nicolás Fissolo, Xavier Montalban, Manuel Comabella

Producció científica: Contribució a una revistaArticleRecercaAvaluat per experts

2 Cites (Scopus)


Multiple sclerosis (MS) is the most common inflammatory, demyelinating, and neurodegenerative disorder of the central nervous system (CNS) in humans. Although the etiology of MS remains unknown, several lines of evidence support the notion that autoimmunity against components of the myelin sheath plays a major role in susceptibility to and development of the disease. At present, there are no approved MS therapies aimed specifically toward downregulating antigen-specific autoreactive immune cells. One antigen-specific approach that appears promising for the treatment of MS is DNA vaccination. This technique has demonstrated efficacy in clinical trials while maintaining safety. Here, we describe the generation of DNA vaccines containing immunologically relevant antigens of MS. Moreover, we present a detailed protocol for the prophylactic and therapeutic administration of DNA vaccines via intramuscular injection targeting on the development of experimental autoimmune encephalomyelitis (EAE), an animal model resembling MS.

Idioma originalEnglish
Pàgines (de-a)39-50
Nombre de pàgines12
RevistaMethods in Molecular Biology
Estat de la publicacióPublicada - 2015


Navegar pels temes de recerca de 'DNA vaccination techniques'. Junts formen un fingerprint únic.

Com citar-ho