TY - JOUR
T1 - Disposable multiple internal reflection systems for photonic cell screening
AU - Ibarlucea, B.
AU - Vila-Planas, J.
AU - Fernández-Rosas, E.
AU - Demming, S.
AU - Nogues, C.
AU - Plaza, J. A.
AU - Büttgenbach, S.
AU - Llobera, A.
PY - 2010
Y1 - 2010
N2 - A low-cost photonic lab on a chip with three different working regimes for cell screening is presented, namely scattering, scattering+absorbance and absorbance. In all regimes, the whole spectral response in absorbance is obtained, being possible to determine for each regime which is the optimal working wavelength that provides the highest performance. Experimental limits of detection (LOD) of 54.9 ± 0.7 cells, 53 ± 1 cells, and 105 ± 4 cells, depending on the working regime used, have been obtained. The system has also been used for measuring the dead/live cell ratio, obtaining LODs between 7.6 ± 0.4% and 6.7 ± 0.3% of dead cells.
AB - A low-cost photonic lab on a chip with three different working regimes for cell screening is presented, namely scattering, scattering+absorbance and absorbance. In all regimes, the whole spectral response in absorbance is obtained, being possible to determine for each regime which is the optimal working wavelength that provides the highest performance. Experimental limits of detection (LOD) of 54.9 ± 0.7 cells, 53 ± 1 cells, and 105 ± 4 cells, depending on the working regime used, have been obtained. The system has also been used for measuring the dead/live cell ratio, obtaining LODs between 7.6 ± 0.4% and 6.7 ± 0.3% of dead cells.
KW - Cell screening
KW - PDMS
KW - Photonic lab-on-a-chip
KW - PMIR
KW - Cell screening
KW - PDMS
KW - Photonic lab-on-a-chip
KW - PMIR
KW - Cell screening
KW - PDMS
KW - Photonic lab-on-a-chip
KW - PMIR
UR - http://www.scopus.com/inward/record.url?scp=84884391335&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:84884391335
VL - 2
SP - 941
EP - 943
JO - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
JF - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
ER -