TY - JOUR
T1 - Development of simple multiplex real-time pcr assays for foodborne pathogens detection and identification on lightcycler
AU - Karus, Avo
AU - Ceciliani, Fabrizio
AU - Bonastre, Armand Sanches
AU - Karus, Virge
PY - 2017/1/1
Y1 - 2017/1/1
N2 - © 2017 Karus A. Most acute intestinal diseases are caused by food-borne pathogens. A fast and simple real-time PCR-based procedure for simultaneous detection of food contamination by any of the five food-borne pathogens: Campylobacter jejuni, Mycobacterium bovis, Enterobacter sakazaki, Shigella boydii, Clostridium perfrigens using multiplex EvaGreen real-time PCR for LightCycler was developed and evaluated. Real-time qPCR showed excellent sensitivity. Tm calling and Melting Curve Genotyping (MCG) were used for analysis of PCR product melting curves. The Melting Curve Genotyping option showed good performance for discrimination of positive samples containing DNA of single pathogen or pathogen mixtures from negative samples.
AB - © 2017 Karus A. Most acute intestinal diseases are caused by food-borne pathogens. A fast and simple real-time PCR-based procedure for simultaneous detection of food contamination by any of the five food-borne pathogens: Campylobacter jejuni, Mycobacterium bovis, Enterobacter sakazaki, Shigella boydii, Clostridium perfrigens using multiplex EvaGreen real-time PCR for LightCycler was developed and evaluated. Real-time qPCR showed excellent sensitivity. Tm calling and Melting Curve Genotyping (MCG) were used for analysis of PCR product melting curves. The Melting Curve Genotyping option showed good performance for discrimination of positive samples containing DNA of single pathogen or pathogen mixtures from negative samples.
KW - Food-borne pathogens
KW - Melting curve genotyping
KW - Multiplex real-time PCR
U2 - 10.1515/macvetrev-2017-0010
DO - 10.1515/macvetrev-2017-0010
M3 - Article
SN - 1409-7621
VL - 40
SP - 53
EP - 58
JO - Macedonian Veterinary Review
JF - Macedonian Veterinary Review
IS - 1
ER -