TY - JOUR
T1 - Detection of the administration of 17β-nortestosterone in boars by gas chromatography/mass spectrometry
AU - Ventura, Rosa
AU - Roig, Meritxell
AU - Pérez, Belén
AU - López, Sergi
AU - Medina, Mireia
AU - Bosch, Jaume
AU - Segura, Jordi
PY - 2008/1/1
Y1 - 2008/1/1
N2 - 17β-Nortestosterone (17βN) is illegally used in livestock as a growth promoter and its endogenous production has been described in some animals, such as adult boars. In this paper, the metabolism of 17βN in boars has been studied by gas chromatography/mass spectrometry (GC/MS) in order to identify markers of the exogenous administration. Administration studies of intramuscular 17βN laurate to male pigs were performed. Free, sulphate and glucuronide fractions of the urine samples were separated and the steroids present were quantified by GC/MS. 17βN was detected in some pre-administration samples. After administration, 17βN, norandrosterone, noretiocholanolone (NorE), norepiandrosterone, 5β-estrane-3α, 17β-diol and 5α-estrane-3β,17β-diol were detected in different fractions, being the most important metabolites, 17βN excreted as a sulphate and free NorE. Samples collected in routine controls were also analyzed by GC/MS to identify endogenous compounds. 17βN, norandrostenedione and estrone were detected in almost all the samples. No other 17βN metabolites were detected. According to these results, the detection by GC/MS of some of the 17βN metabolites described above, different from 17βN, could be indicative of the exogenous administration of 17βN to boars. Copyright © 2008 John Wiley & Sons, Ltd.
AB - 17β-Nortestosterone (17βN) is illegally used in livestock as a growth promoter and its endogenous production has been described in some animals, such as adult boars. In this paper, the metabolism of 17βN in boars has been studied by gas chromatography/mass spectrometry (GC/MS) in order to identify markers of the exogenous administration. Administration studies of intramuscular 17βN laurate to male pigs were performed. Free, sulphate and glucuronide fractions of the urine samples were separated and the steroids present were quantified by GC/MS. 17βN was detected in some pre-administration samples. After administration, 17βN, norandrosterone, noretiocholanolone (NorE), norepiandrosterone, 5β-estrane-3α, 17β-diol and 5α-estrane-3β,17β-diol were detected in different fractions, being the most important metabolites, 17βN excreted as a sulphate and free NorE. Samples collected in routine controls were also analyzed by GC/MS to identify endogenous compounds. 17βN, norandrostenedione and estrone were detected in almost all the samples. No other 17βN metabolites were detected. According to these results, the detection by GC/MS of some of the 17βN metabolites described above, different from 17βN, could be indicative of the exogenous administration of 17βN to boars. Copyright © 2008 John Wiley & Sons, Ltd.
U2 - 10.1002/rcm.3563
DO - 10.1002/rcm.3563
M3 - Article
SN - 0951-4198
VL - 22
SP - 1863
EP - 1870
JO - Rapid Communications in Mass Spectrometry
JF - Rapid Communications in Mass Spectrometry
ER -