TY - JOUR
T1 - Detection of circulating cancer cells using electrocatalytic gold nanoparticles
AU - Maltez-Da Costa, Marisa
AU - De La Escosura-Muñiz, Alfredo
AU - Nogués, Carme
AU - Barrios, Lleonard
AU - Ibáñez, Elena
AU - Merkoçi, Arben
PY - 2012/12/7
Y1 - 2012/12/7
N2 - A rapid cancer cell detection and quantification assay, based on the electrocatalytic properties of gold nanoparticles towards the hydrogen evolution reaction, is described. The selective labeling of cancer cells is performed in suspension, allowing a fast interaction between the gold nanoparticle labels and the target proteins expressed at the cell membrane. The subsequent electrochemical detection is accomplished with small volumes of sample and user-friendly equipment through a simple electrochemical method that generates a fast electrochemical response used for the quantification of nanoparticle-labeled cancer cells. The system establishes a selective cell-detection assay capable of detecting 4 × 103 cancer cells in suspension that can be extended to several other cells detection scenarios. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
AB - A rapid cancer cell detection and quantification assay, based on the electrocatalytic properties of gold nanoparticles towards the hydrogen evolution reaction, is described. The selective labeling of cancer cells is performed in suspension, allowing a fast interaction between the gold nanoparticle labels and the target proteins expressed at the cell membrane. The subsequent electrochemical detection is accomplished with small volumes of sample and user-friendly equipment through a simple electrochemical method that generates a fast electrochemical response used for the quantification of nanoparticle-labeled cancer cells. The system establishes a selective cell-detection assay capable of detecting 4 × 103 cancer cells in suspension that can be extended to several other cells detection scenarios. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
KW - cancer diagnosis
KW - circulating cancer cells
KW - electrocatalytic properties
KW - gold nanoparticles
KW - hydrogen evolution reaction
UR - https://www.scopus.com/pages/publications/84870598356
U2 - 10.1002/smll.201201205
DO - 10.1002/smll.201201205
M3 - Article
SN - 1613-6810
VL - 8
SP - 3605
EP - 3612
JO - Small
JF - Small
ER -
