Detection and purification of bovine herpesvirus 1 glycoproteins by lectin affinity

Mercè Badia, Enrique Querol

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Resum

A procedure for detection and purification of bovine herpesvirus 1 glycoproteins by their affinity for lectins is described. Wheat germ lectin, Limulus polyphemus lectin and concanavalin A, radioactive or biotin-labelled, have been used for the in vitro characterization of the glycoproteins. These lectins have also been used for affinity-purification of sets of glycoproteins. The procedure is easy, rapid, cheap and, when biotin is used, non-radioactive. © 1988.
Idioma originalAnglès
Pàgines (de-a)23-29
RevistaJournal of Virological Methods
Volum22
Número1
DOIs
Estat de la publicacióPublicada - 1 de gen. 1988

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