Deep Sequencing of RNA from Ancient Maize Kernels

Sarah L. Fordyce; Maria C. Ávila-Arcos; Morten Rasmussen; Enrico Cappellini; J. Alberto Romero-Navarro; Nathan Wales; David E. Alquezar-Planas; Steven Penfield; Terence A. Brown; Jean-Philippe Vielle-Calzada; Rafael Montiel; Tina Jørgensen; Nancy Odegaard; Michael Jacobs; Bernardo Arriaza; Thomas F. G. Higham; Christopher Bronk Ramsey; Eske Willerslev; M. Thomas P. Gilbert; Dorian Q. Fuller

doi:10.1371/journal.pone.0050961

Deep Sequencing of RNA from Ancient Maize Kernels

Sarah L. Fordyce, Maria C. Ávila-Arcos, Morten Rasmussen, Enrico Cappellini, J. Alberto Romero-Navarro, Nathan Wales, David E. Alquezar-Planas, Steven Penfield, Terence A. Brown, Jean-Philippe Vielle-Calzada, Rafael Montiel, Tina Jørgensen, Nancy Odegaard, Michael Jacobs, Bernardo Arriaza, Thomas F. G. Higham, Christopher Bronk Ramsey, Eske Willerslev, M. Thomas P. Gilbert, Dorian Q. Fuller (Editor)

Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia

Producció científica: Contribució a revista › Article › Recerca › Avaluat per experts

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Resum

The characterization of biomolecules from ancient samples can shed otherwise unobtainable insights into the past. Despite the fundamental role of transcriptomal change in evolution, the potential of ancient RNA remains unexploited – perhaps due to dogma associated with the fragility of RNA. We hypothesize that seeds offer a plausible refuge for long-term RNA survival, due to the fundamental role of RNA during seed germination. Using RNA-Seq on cDNA synthesized from nucleic acid extracts, we validate this hypothesis through demonstration of partial transcriptomal recovery from two sources of ancient maize kernels. The results suggest that ancient seed transcriptomics may offer a powerful new tool with which to study plant domestication.

Idioma original	Anglès
Número d’article	e50961
Revista	PloS one
Volum	8
Número	1
DOIs	https://doi.org/10.1371/journal.pone.0050961
Estat de la publicació	Publicada - 11 de gen. 2013

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10.1371/journal.pone.0050961

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Fordyce, S. L., Ávila-Arcos, M. C., Rasmussen, M., Cappellini, E., Romero-Navarro, J. A., Wales, N., Alquezar-Planas, D. E., Penfield, S., Brown, T. A., Vielle-Calzada, J.-P., Montiel, R., Jørgensen, T., Odegaard, N., Jacobs, M., Arriaza, B., Higham, T. F. G., Ramsey, C. B., Willerslev, E., Gilbert, M. T. P., & Fuller, D. Q. (Ed.) (2013). Deep Sequencing of RNA from Ancient Maize Kernels. PloS one, 8(1), Article e50961. https://doi.org/10.1371/journal.pone.0050961

@article{f69a5f06634148b88e3cffd78f89c8c6,

title = "Deep Sequencing of RNA from Ancient Maize Kernels",

abstract = "The characterization of biomolecules from ancient samples can shed otherwise unobtainable insights into the past. Despite the fundamental role of transcriptomal change in evolution, the potential of ancient RNA remains unexploited – perhaps due to dogma associated with the fragility of RNA. We hypothesize that seeds offer a plausible refuge for long-term RNA survival, due to the fundamental role of RNA during seed germination. Using RNA-Seq on cDNA synthesized from nucleic acid extracts, we validate this hypothesis through demonstration of partial transcriptomal recovery from two sources of ancient maize kernels. The results suggest that ancient seed transcriptomics may offer a powerful new tool with which to study plant domestication.",

author = "Fordyce, {Sarah L.} and {\'A}vila-Arcos, {Maria C.} and Morten Rasmussen and Enrico Cappellini and Romero-Navarro, {J. Alberto} and Nathan Wales and Alquezar-Planas, {David E.} and Steven Penfield and Brown, {Terence A.} and Jean-Philippe Vielle-Calzada and Rafael Montiel and Tina J{\o}rgensen and Nancy Odegaard and Michael Jacobs and Bernardo Arriaza and Higham, {Thomas F. G.} and Ramsey, {Christopher Bronk} and Eske Willerslev and Gilbert, {M. Thomas P.} and Fuller, {Dorian Q.}",

year = "2013",

month = jan,

day = "11",

doi = "10.1371/journal.pone.0050961",

language = "English",

volume = "8",

number = "1",

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Fordyce, SL, Ávila-Arcos, MC, Rasmussen, M, Cappellini, E, Romero-Navarro, JA, Wales, N, Alquezar-Planas, DE, Penfield, S, Brown, TA, Vielle-Calzada, J-P, Montiel, R, Jørgensen, T, Odegaard, N, Jacobs, M, Arriaza, B, Higham, TFG, Ramsey, CB, Willerslev, E, Gilbert, MTP & Fuller, DQ (ed.) 2013, 'Deep Sequencing of RNA from Ancient Maize Kernels', PloS one, vol. 8, núm. 1, e50961. https://doi.org/10.1371/journal.pone.0050961

TY - JOUR

T1 - Deep Sequencing of RNA from Ancient Maize Kernels

AU - Fordyce, Sarah L.

AU - Ávila-Arcos, Maria C.

AU - Rasmussen, Morten

AU - Cappellini, Enrico

AU - Romero-Navarro, J. Alberto

AU - Wales, Nathan

AU - Alquezar-Planas, David E.

AU - Penfield, Steven

AU - Brown, Terence A.

AU - Vielle-Calzada, Jean-Philippe

AU - Montiel, Rafael

AU - Jørgensen, Tina

AU - Odegaard, Nancy

AU - Jacobs, Michael

AU - Arriaza, Bernardo

AU - Higham, Thomas F. G.

AU - Ramsey, Christopher Bronk

AU - Willerslev, Eske

AU - Gilbert, M. Thomas P.

A2 - Fuller, Dorian Q.

PY - 2013/1/11

Y1 - 2013/1/11

N2 - The characterization of biomolecules from ancient samples can shed otherwise unobtainable insights into the past. Despite the fundamental role of transcriptomal change in evolution, the potential of ancient RNA remains unexploited – perhaps due to dogma associated with the fragility of RNA. We hypothesize that seeds offer a plausible refuge for long-term RNA survival, due to the fundamental role of RNA during seed germination. Using RNA-Seq on cDNA synthesized from nucleic acid extracts, we validate this hypothesis through demonstration of partial transcriptomal recovery from two sources of ancient maize kernels. The results suggest that ancient seed transcriptomics may offer a powerful new tool with which to study plant domestication.

AB - The characterization of biomolecules from ancient samples can shed otherwise unobtainable insights into the past. Despite the fundamental role of transcriptomal change in evolution, the potential of ancient RNA remains unexploited – perhaps due to dogma associated with the fragility of RNA. We hypothesize that seeds offer a plausible refuge for long-term RNA survival, due to the fundamental role of RNA during seed germination. Using RNA-Seq on cDNA synthesized from nucleic acid extracts, we validate this hypothesis through demonstration of partial transcriptomal recovery from two sources of ancient maize kernels. The results suggest that ancient seed transcriptomics may offer a powerful new tool with which to study plant domestication.

U2 - 10.1371/journal.pone.0050961

DO - 10.1371/journal.pone.0050961

M3 - Article

SN - 1932-6203

VL - 8

JO - PloS one

JF - PloS one

IS - 1

M1 - e50961

ER -

Deep Sequencing of RNA from Ancient Maize Kernels

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