Complementary positional proteomics for screening substrates of endo-and exoproteases

Petra Van Damme; An Staes; Silvia Bronsoms; Kenny Helsens; Niklaas Colaert; Evy Timmerman; Francesc X. Aviles; Joël Vandekerckhove; Kris Gevaert

doi:10.1038/nmeth.1469

Complementary positional proteomics for screening substrates of endo-and exoproteases

Petra Van Damme, An Staes, Silvia Bronsoms, Kenny Helsens, Niklaas Colaert, Evy Timmerman, Francesc X. Aviles, Joël Vandekerckhove, Kris Gevaert

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We describe a positional proteomics approach to simultaneously analyze N-and C-terminal peptides and used it to screen for human protein substrates of granzyme B and carboxypeptidase A4 in human cell lysates. This approach allowed comprehensive proteome studies, and we report the identification of 965 database-annotated protein C termini, 334 neo-C termini resulting from granzyme B processing and 16 neo-C termini resulting from carboxypeptidase A4 processing. © 2010 Nature America, Inc. All rights reserved.

Idioma original	Anglès
Pàgines (de-a)	512-515
Revista	Nature Methods
Volum	7
DOIs	https://doi.org/10.1038/nmeth.1469
Estat de la publicació	Publicada - 1 de jul. 2010

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title = "Complementary positional proteomics for screening substrates of endo-and exoproteases",

abstract = "We describe a positional proteomics approach to simultaneously analyze N-and C-terminal peptides and used it to screen for human protein substrates of granzyme B and carboxypeptidase A4 in human cell lysates. This approach allowed comprehensive proteome studies, and we report the identification of 965 database-annotated protein C termini, 334 neo-C termini resulting from granzyme B processing and 16 neo-C termini resulting from carboxypeptidase A4 processing. {\textcopyright} 2010 Nature America, Inc. All rights reserved.",

author = "\{Van Damme\}, Petra and An Staes and Silvia Bronsoms and Kenny Helsens and Niklaas Colaert and Evy Timmerman and Aviles, \{Francesc X.\} and Jo{\"e}l Vandekerckhove and Kris Gevaert",

year = "2010",

month = jul,

day = "1",

doi = "10.1038/nmeth.1469",

language = "English",

volume = "7",

pages = "512--515",

journal = "Nature Methods",

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TY - JOUR

T1 - Complementary positional proteomics for screening substrates of endo-and exoproteases

AU - Van Damme, Petra

AU - Staes, An

AU - Bronsoms, Silvia

AU - Helsens, Kenny

AU - Colaert, Niklaas

AU - Timmerman, Evy

AU - Aviles, Francesc X.

AU - Vandekerckhove, Joël

AU - Gevaert, Kris

PY - 2010/7/1

Y1 - 2010/7/1

N2 - We describe a positional proteomics approach to simultaneously analyze N-and C-terminal peptides and used it to screen for human protein substrates of granzyme B and carboxypeptidase A4 in human cell lysates. This approach allowed comprehensive proteome studies, and we report the identification of 965 database-annotated protein C termini, 334 neo-C termini resulting from granzyme B processing and 16 neo-C termini resulting from carboxypeptidase A4 processing. © 2010 Nature America, Inc. All rights reserved.

AB - We describe a positional proteomics approach to simultaneously analyze N-and C-terminal peptides and used it to screen for human protein substrates of granzyme B and carboxypeptidase A4 in human cell lysates. This approach allowed comprehensive proteome studies, and we report the identification of 965 database-annotated protein C termini, 334 neo-C termini resulting from granzyme B processing and 16 neo-C termini resulting from carboxypeptidase A4 processing. © 2010 Nature America, Inc. All rights reserved.

UR - https://www.scopus.com/pages/publications/77954694885

U2 - 10.1038/nmeth.1469

DO - 10.1038/nmeth.1469

M3 - Article

SN - 1548-7091

VL - 7

SP - 512

EP - 515

JO - Nature Methods

JF - Nature Methods

ER -

Complementary positional proteomics for screening substrates of endo-and exoproteases

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