Comparison of muscle fluid and serum for detection of antibodies against hepatitis E virus in slaughter pigs

Maribel Casas; Sonia Pina; Bibiana Peralta; Enric Mateu; Jordi Casal; Marga Martín

doi:10.1016/j.tvjl.2010.09.030

Comparison of muscle fluid and serum for detection of antibodies against hepatitis E virus in slaughter pigs

Maribel Casas, Sonia Pina, Bibiana Peralta, Enric Mateu, Jordi Casal, Marga Martín

Departament de Sanitat i d'Anatomia Animals

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Muscle fluid was investigated as an alternative to serum for detecting anti-hepatitis E virus (HEV) antibodies in slaughter pigs. Samples of serum and diaphragmatic muscle juice from 67 pigs were analysed by anti-HEV IgG ELISA. Compared to the serum ELISA, the ELISA on diaphragmatic muscle fluid had a sensitivity of 93%, a specificity of 91.7%, a κ value of 0.84 and a determination coefficient (R 2) of 0.77; both tests had global agreement of results. Muscle fluid can be used as an alternative to serum for serological detection of HEV antibodies in slaughter pigs. © 2010 Elsevier Ltd.

Idioma original	Anglès
Pàgines (de-a)	179-180
Revista	Veterinary Journal
Volum	190
DOIs	https://doi.org/10.1016/j.tvjl.2010.09.030
Estat de la publicació	Publicada - 1 d’oct. 2011

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10.1016/j.tvjl.2010.09.030

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title = "Comparison of muscle fluid and serum for detection of antibodies against hepatitis E virus in slaughter pigs",

abstract = "Muscle fluid was investigated as an alternative to serum for detecting anti-hepatitis E virus (HEV) antibodies in slaughter pigs. Samples of serum and diaphragmatic muscle juice from 67 pigs were analysed by anti-HEV IgG ELISA. Compared to the serum ELISA, the ELISA on diaphragmatic muscle fluid had a sensitivity of 93%, a specificity of 91.7%, a κ value of 0.84 and a determination coefficient (R 2) of 0.77; both tests had global agreement of results. Muscle fluid can be used as an alternative to serum for serological detection of HEV antibodies in slaughter pigs. {\textcopyright} 2010 Elsevier Ltd.",

keywords = "ELISA, Hepatitis E virus, Muscle fluid, Pigs, Serology",

author = "Maribel Casas and Sonia Pina and Bibiana Peralta and Enric Mateu and Jordi Casal and Marga Mart{\'i}n",

year = "2011",

month = oct,

day = "1",

doi = "10.1016/j.tvjl.2010.09.030",

language = "English",

volume = "190",

pages = "179--180",

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TY - JOUR

T1 - Comparison of muscle fluid and serum for detection of antibodies against hepatitis E virus in slaughter pigs

AU - Casas, Maribel

AU - Pina, Sonia

AU - Peralta, Bibiana

AU - Mateu, Enric

AU - Casal, Jordi

AU - Martín, Marga

PY - 2011/10/1

Y1 - 2011/10/1

N2 - Muscle fluid was investigated as an alternative to serum for detecting anti-hepatitis E virus (HEV) antibodies in slaughter pigs. Samples of serum and diaphragmatic muscle juice from 67 pigs were analysed by anti-HEV IgG ELISA. Compared to the serum ELISA, the ELISA on diaphragmatic muscle fluid had a sensitivity of 93%, a specificity of 91.7%, a κ value of 0.84 and a determination coefficient (R 2) of 0.77; both tests had global agreement of results. Muscle fluid can be used as an alternative to serum for serological detection of HEV antibodies in slaughter pigs. © 2010 Elsevier Ltd.

AB - Muscle fluid was investigated as an alternative to serum for detecting anti-hepatitis E virus (HEV) antibodies in slaughter pigs. Samples of serum and diaphragmatic muscle juice from 67 pigs were analysed by anti-HEV IgG ELISA. Compared to the serum ELISA, the ELISA on diaphragmatic muscle fluid had a sensitivity of 93%, a specificity of 91.7%, a κ value of 0.84 and a determination coefficient (R 2) of 0.77; both tests had global agreement of results. Muscle fluid can be used as an alternative to serum for serological detection of HEV antibodies in slaughter pigs. © 2010 Elsevier Ltd.

KW - ELISA

KW - Hepatitis E virus

KW - Muscle fluid

KW - Pigs

KW - Serology

U2 - 10.1016/j.tvjl.2010.09.030

DO - 10.1016/j.tvjl.2010.09.030

M3 - Article

SN - 1090-0233

VL - 190

SP - 179

EP - 180

JO - Veterinary Journal

JF - Veterinary Journal

ER -

Comparison of muscle fluid and serum for detection of antibodies against hepatitis E virus in slaughter pigs

Resum

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