TY - JOUR
T1 - Combination of extended X-ray absorption fine structure spectroscopy with lipidic cubic phases for the study of cation binding in bacteriorhodopsin
AU - Perálvarez-Marín, Alex
AU - Sepulcre, Francesc
AU - Márquez, Mercedes
AU - Proietti, Maria Grazia
AU - Padrós, Esteve
PY - 2011/8/1
Y1 - 2011/8/1
N2 - We have performed a quantitative X-ray absorption fine structure analysis of bacteriorhodopsin in purple membrane patches and in lipidic cubic phases regenerated with Mn 2+. Lipidic cubic phases and purple membrane results have been compared, demonstrating that the lipidic cubic phase process does not introduce relevant distortions in the local geometry of the cation binding sites. For both samples, we have observed similarities for Mn 2+ coordination in terms of type, number, and average distances of surrounding atoms, indicating a first coordination shell composed by 6 O atoms, and 3/4 C atoms located in the second coordination shell. © 2011 European Biophysical Societies' Association.
AB - We have performed a quantitative X-ray absorption fine structure analysis of bacteriorhodopsin in purple membrane patches and in lipidic cubic phases regenerated with Mn 2+. Lipidic cubic phases and purple membrane results have been compared, demonstrating that the lipidic cubic phase process does not introduce relevant distortions in the local geometry of the cation binding sites. For both samples, we have observed similarities for Mn 2+ coordination in terms of type, number, and average distances of surrounding atoms, indicating a first coordination shell composed by 6 O atoms, and 3/4 C atoms located in the second coordination shell. © 2011 European Biophysical Societies' Association.
KW - Bacteriorhodopsin
KW - Cation binding
KW - EXAFS
KW - Lipidic cubic phase
KW - Membrane protein
U2 - 10.1007/s00249-011-0714-3
DO - 10.1007/s00249-011-0714-3
M3 - Article
SN - 0175-7571
VL - 40
SP - 1007
EP - 1012
JO - European Biophysics Journal
JF - European Biophysics Journal
IS - 8
ER -