TY - JOUR
T1 - Collaborative Evaluation of Optimal Antifungal Susceptibility Testing Conditions for Dermatophytes
AU - Fernández-Torres, B.
AU - Cabañes, F.J.
AU - Carrillo-Muñoz, A.J.
AU - Esteban, A.
AU - Inza, I.
AU - Abarca, M.L.
AU - Guarro, J.
PY - 2002
Y1 - 2002
N2 - A multicenter study was conducted to define the most suitable testing conditions for antifungal susceptibility of dermatophytes. Broth microdilution MICs of clotrimazole, itraconazole, and terbinafine were determined in three centers against 60 strains of dermatophytes. The effects of inoculum density (ca. 103 and 104 CFU/ml), incubation time (3, 7, and 14 days), endpoint criteria for MIC determination (complete [MIC-0] and prominent [MIC-2] growth inhibition), and incubation temperature (28 and 37°C) on intra- and interlaboratory agreement were analyzed. The optimal testing conditions identified were an inoculum of 104 CFU/ml, a temperature of incubation of 28°C, an incubation period of 7 days, and MIC-0.
AB - A multicenter study was conducted to define the most suitable testing conditions for antifungal susceptibility of dermatophytes. Broth microdilution MICs of clotrimazole, itraconazole, and terbinafine were determined in three centers against 60 strains of dermatophytes. The effects of inoculum density (ca. 103 and 104 CFU/ml), incubation time (3, 7, and 14 days), endpoint criteria for MIC determination (complete [MIC-0] and prominent [MIC-2] growth inhibition), and incubation temperature (28 and 37°C) on intra- and interlaboratory agreement were analyzed. The optimal testing conditions identified were an inoculum of 104 CFU/ml, a temperature of incubation of 28°C, an incubation period of 7 days, and MIC-0.
KW - Micologia Veterinària
U2 - 10.1128/jcm.40.11.3999-4003.2002
DO - 10.1128/jcm.40.11.3999-4003.2002
M3 - Article
C2 - 12409365
SN - 0095-1137
VL - 40
SP - 3999
EP - 4003
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 11
ER -