Collaborative Evaluation of Optimal Antifungal Susceptibility Testing Conditions for Dermatophytes

B. Fernández-Torres, F.J. Cabañes, A.J. Carrillo-Muñoz, A. Esteban, I. Inza, M.L. Abarca, J. Guarro

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Resum

A multicenter study was conducted to define the most suitable testing conditions for antifungal susceptibility of dermatophytes. Broth microdilution MICs of clotrimazole, itraconazole, and terbinafine were determined in three centers against 60 strains of dermatophytes. The effects of inoculum density (ca. 103 and 104 CFU/ml), incubation time (3, 7, and 14 days), endpoint criteria for MIC determination (complete [MIC-0] and prominent [MIC-2] growth inhibition), and incubation temperature (28 and 37°C) on intra- and interlaboratory agreement were analyzed. The optimal testing conditions identified were an inoculum of 104 CFU/ml, a temperature of incubation of 28°C, an incubation period of 7 days, and MIC-0.
Idioma originalAnglès
Pàgines (de-a)3999-4003
Nombre de pàgines5
RevistaJournal of Clinical Microbiology
Volum40
Número11
DOIs
Estat de la publicacióPublicada - 2002

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