Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing

Marian Garcia-Nuñez; Sara Quero; Maria Lluisa Pedro-Botet; Irene Barrabeig; Josep Avarez; Irene Campoy; Maria Rosa Sala; Noemí Parraga-Niño; Sofia Minguell; Joan Caylà; Lourdes Mateu; Miquel Sabria

doi:10.2217/fmb-2016-0013

Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing

Marian Garcia-Nuñez, Sara Quero, Maria Lluisa Pedro-Botet, Irene Barrabeig, Josep Avarez, Irene Campoy, Maria Rosa Sala, Noemí Parraga-Niño, Sofia Minguell, Joan Caylà, Lourdes Mateu, Miquel Sabria

Departament de Medicina

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© 2016 Future Medicine Ltd. Aim: To characterize the genetic diversity of unrelated Legionella pneumophila clinical isolates in Catalonia and compare with other European regions. Methods: 95 unrelated isolates were analyzed using monoclonal antibodies and sequence-based typing, 1989-2013. Results: The isolates showed a high diversity (IOD 0.964) with a predominance of some profiles (ST37-Phialdelphia, ST23-Philadelphia and ST1-OLDA). All regions had predominant sequence types (STs) that differed between regions, and only 3% of STs were shared between the three regions. Conclusion: L. pneumophila clinical isolates from Catalonia presented a high diversity and can be used in epidemiological surveillance studies. The heterogeneous predominance of STs between European regions suggested a relationship between geographical distribution and virulence of some STs.

Idioma original	Anglès
Pàgines (de-a)	865-875
Revista	Future Microbiology
Volum	11
Número	7
DOIs	https://doi.org/10.2217/fmb-2016-0013
Estat de la publicació	Publicada - 1 de jul. 2016

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10.2217/fmb-2016-0013

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Garcia-Nuñez, M., Quero, S., Pedro-Botet, M. L., Barrabeig, I., Avarez, J., Campoy, I., Sala, M. R., Parraga-Niño, N., Minguell, S., Caylà, J., Mateu, L., & Sabria, M. (2016). Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing. Future Microbiology, 11(7), 865-875. https://doi.org/10.2217/fmb-2016-0013

@article{7330529516eb4ec898ce864acf041465,

title = "Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing",

abstract = "{\textcopyright} 2016 Future Medicine Ltd. Aim: To characterize the genetic diversity of unrelated Legionella pneumophila clinical isolates in Catalonia and compare with other European regions. Methods: 95 unrelated isolates were analyzed using monoclonal antibodies and sequence-based typing, 1989-2013. Results: The isolates showed a high diversity (IOD 0.964) with a predominance of some profiles (ST37-Phialdelphia, ST23-Philadelphia and ST1-OLDA). All regions had predominant sequence types (STs) that differed between regions, and only 3% of STs were shared between the three regions. Conclusion: L. pneumophila clinical isolates from Catalonia presented a high diversity and can be used in epidemiological surveillance studies. The heterogeneous predominance of STs between European regions suggested a relationship between geographical distribution and virulence of some STs.",

keywords = "clinical isolates, Legionella, monoclonal antibody, sequence-based typing",

author = "Marian Garcia-Nu{\~n}ez and Sara Quero and Pedro-Botet, {Maria Lluisa} and Irene Barrabeig and Josep Avarez and Irene Campoy and Sala, {Maria Rosa} and Noem{\'i} Parraga-Ni{\~n}o and Sofia Minguell and Joan Cayl{\`a} and Lourdes Mateu and Miquel Sabria",

year = "2016",

month = jul,

day = "1",

doi = "10.2217/fmb-2016-0013",

language = "English",

volume = "11",

pages = "865--875",

journal = "Future Microbiology",

issn = "1746-0913",

publisher = "Future Medicine Ltd.",

number = "7",

}

Garcia-Nuñez, M, Quero, S, Pedro-Botet, ML, Barrabeig, I, Avarez, J, Campoy, I, Sala, MR, Parraga-Niño, N, Minguell, S, Caylà, J, Mateu, L & Sabria, M 2016, 'Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing', Future Microbiology, vol. 11, núm. 7, pàg. 865-875. https://doi.org/10.2217/fmb-2016-0013

Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing. / Garcia-Nuñez, Marian; Quero, Sara; Pedro-Botet, Maria Lluisa et al.
In: Future Microbiology, Vol. 11, Núm. 7, 01.07.2016, pàg. 865-875.

Producció científica: Contribució a revista › Article › Recerca › Avaluat per experts

TY - JOUR

T1 - Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing

AU - Garcia-Nuñez, Marian

AU - Quero, Sara

AU - Pedro-Botet, Maria Lluisa

AU - Barrabeig, Irene

AU - Avarez, Josep

AU - Campoy, Irene

AU - Sala, Maria Rosa

AU - Parraga-Niño, Noemí

AU - Minguell, Sofia

AU - Caylà, Joan

AU - Mateu, Lourdes

AU - Sabria, Miquel

PY - 2016/7/1

Y1 - 2016/7/1

N2 - © 2016 Future Medicine Ltd. Aim: To characterize the genetic diversity of unrelated Legionella pneumophila clinical isolates in Catalonia and compare with other European regions. Methods: 95 unrelated isolates were analyzed using monoclonal antibodies and sequence-based typing, 1989-2013. Results: The isolates showed a high diversity (IOD 0.964) with a predominance of some profiles (ST37-Phialdelphia, ST23-Philadelphia and ST1-OLDA). All regions had predominant sequence types (STs) that differed between regions, and only 3% of STs were shared between the three regions. Conclusion: L. pneumophila clinical isolates from Catalonia presented a high diversity and can be used in epidemiological surveillance studies. The heterogeneous predominance of STs between European regions suggested a relationship between geographical distribution and virulence of some STs.

AB - © 2016 Future Medicine Ltd. Aim: To characterize the genetic diversity of unrelated Legionella pneumophila clinical isolates in Catalonia and compare with other European regions. Methods: 95 unrelated isolates were analyzed using monoclonal antibodies and sequence-based typing, 1989-2013. Results: The isolates showed a high diversity (IOD 0.964) with a predominance of some profiles (ST37-Phialdelphia, ST23-Philadelphia and ST1-OLDA). All regions had predominant sequence types (STs) that differed between regions, and only 3% of STs were shared between the three regions. Conclusion: L. pneumophila clinical isolates from Catalonia presented a high diversity and can be used in epidemiological surveillance studies. The heterogeneous predominance of STs between European regions suggested a relationship between geographical distribution and virulence of some STs.

KW - clinical isolates

KW - Legionella

KW - monoclonal antibody

KW - sequence-based typing

U2 - 10.2217/fmb-2016-0013

DO - 10.2217/fmb-2016-0013

M3 - Article

SN - 1746-0913

VL - 11

SP - 865

EP - 875

JO - Future Microbiology

JF - Future Microbiology

IS - 7

ER -

Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing

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