TY - JOUR
T1 - Cerium oxide nanoparticles display antilipogenic effect in rats with non-alcoholic fatty liver disease
AU - Carvajal, Silvia
AU - Perramón, Meritxell
AU - Oró, Denise
AU - Casals, Eudald
AU - Fernández-Varo, Guillermo
AU - Parra-Robert, Marina
AU - González de la Presa, Bernardino.
AU - Ribera, Jordi
AU - Pastor, Óscar
AU - Morales Ruiz, Manuel
AU - Puntes, Víctor
AU - Jiménez, Wladimiro
PY - 2019
Y1 - 2019
N2 - Non-alcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease worldwide, ranging from steatosis to non-alcoholic steatohepatitis (NASH). Recently, cerium oxide nanoparticles (CeO₂NPs) have emerged as a new antioxidant agent with hepatoprotective properties in experimental liver disease. The aim of the current investigation was to elucidate whether CeO₂NPs display beneficial effects in an experimental model of NAFLD.Therefore, fifteen Wistar rats were subjected to a methionine and choline deficient diet (MCDD) for 6 weeks and intravenously treated with CeO₂NPs or vehicle during the weeks three and four of the diet. The effect of CeO₂NPs on serum biochemistry, hepatic steatosis, inflammation, fatty acid content and expression of reactive oxygen species (ROS) and lipid metabolism related genes was assessed. MCDD fed rats showed increased inflammation, enhanced hepatic lipid accumulation of both saturated and unsaturated fatty acids (FAs) and overexpression of genes related to fatty liver and ROS metabolism. Treatment with CeONPs was able to reduce the size and content of hepatocyte lipid droplets, the hepatic concentration of triglyceride- and cholesterol ester-derived FAs and the expression of several genes involved in cytokine, adipokine and chemokine signaling pathways. These findings suggest that CeO₂NPs could be of beneficial value in NAFLD.
AB - Non-alcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease worldwide, ranging from steatosis to non-alcoholic steatohepatitis (NASH). Recently, cerium oxide nanoparticles (CeO₂NPs) have emerged as a new antioxidant agent with hepatoprotective properties in experimental liver disease. The aim of the current investigation was to elucidate whether CeO₂NPs display beneficial effects in an experimental model of NAFLD.Therefore, fifteen Wistar rats were subjected to a methionine and choline deficient diet (MCDD) for 6 weeks and intravenously treated with CeO₂NPs or vehicle during the weeks three and four of the diet. The effect of CeO₂NPs on serum biochemistry, hepatic steatosis, inflammation, fatty acid content and expression of reactive oxygen species (ROS) and lipid metabolism related genes was assessed. MCDD fed rats showed increased inflammation, enhanced hepatic lipid accumulation of both saturated and unsaturated fatty acids (FAs) and overexpression of genes related to fatty liver and ROS metabolism. Treatment with CeONPs was able to reduce the size and content of hepatocyte lipid droplets, the hepatic concentration of triglyceride- and cholesterol ester-derived FAs and the expression of several genes involved in cytokine, adipokine and chemokine signaling pathways. These findings suggest that CeO₂NPs could be of beneficial value in NAFLD.
KW - Biologics
KW - Nanoparticles
KW - Non-alcoholic fatty liver disease
U2 - 10.1038/s41598-019-49262-2
DO - 10.1038/s41598-019-49262-2
M3 - Article
C2 - 31492960
SN - 2045-2322
VL - 9
JO - Scientific Reports
JF - Scientific Reports
IS - 1
ER -