CD83 expression in sea bream macrophages is a marker for the LPS-induced inflammatory response

C. Doñate; N. Roher; J. C. Balasch; L. Ribas; F. W. Goetz; J. V. Planas; L. Tort; S. MacKenzie

doi:10.1016/j.fsi.2007.03.016

CD83 expression in sea bream macrophages is a marker for the LPS-induced inflammatory response

C. Doñate, N. Roher, J. C. Balasch, L. Ribas, F. W. Goetz, J. V. Planas, L. Tort, S. MacKenzie

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CD83, a cell surface membrane glycoprotein member of the Ig superfamily which is commonly used as standard surface marker for dendritic cells, was cloned from gilthead sea bream macrophages using degenerate primers against conserved motifs of known CD83 sequences. The obtained cDNA contains an open reading frame of 669 nucleotides that translate into a 222 amino acid putative peptide. The deduced protein sequence shows conservation of features shared by vertebrate CD83 and multiple alignment with fish CD83 sequences reveals high homology. In cultured sea bream macrophages CD83 mRNA expression was significantly enhanced in a dose- and time-dependent fashion after stimulation with Escherichia coli LPS. These results indicate that in fish, macrophages express high levels of CD83 mRNA after LPS exposure and CD83 is therefore a good marker for activated mature myeloid cells in fish. © 2007 Elsevier Ltd. All rights reserved.

Idioma original	Anglès
Pàgines (de-a)	877-885
Revista	Fish and Shellfish Immunology
Volum	23
DOIs	https://doi.org/10.1016/j.fsi.2007.03.016
Estat de la publicació	Publicada - 1 de gen. 2007

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10.1016/j.fsi.2007.03.016

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@article{8064dc0997214a1783f2eae4039176f9,

title = "CD83 expression in sea bream macrophages is a marker for the LPS-induced inflammatory response",

abstract = "CD83, a cell surface membrane glycoprotein member of the Ig superfamily which is commonly used as standard surface marker for dendritic cells, was cloned from gilthead sea bream macrophages using degenerate primers against conserved motifs of known CD83 sequences. The obtained cDNA contains an open reading frame of 669 nucleotides that translate into a 222 amino acid putative peptide. The deduced protein sequence shows conservation of features shared by vertebrate CD83 and multiple alignment with fish CD83 sequences reveals high homology. In cultured sea bream macrophages CD83 mRNA expression was significantly enhanced in a dose- and time-dependent fashion after stimulation with Escherichia coli LPS. These results indicate that in fish, macrophages express high levels of CD83 mRNA after LPS exposure and CD83 is therefore a good marker for activated mature myeloid cells in fish. {\textcopyright} 2007 Elsevier Ltd. All rights reserved.",

keywords = "CD83, Fish, LPS, Macrophages, mRNA expression",

author = "C. Do{\~n}ate and N. Roher and Balasch, {J. C.} and L. Ribas and Goetz, {F. W.} and Planas, {J. V.} and L. Tort and S. MacKenzie",

year = "2007",

month = jan,

day = "1",

doi = "10.1016/j.fsi.2007.03.016",

language = "English",

volume = "23",

pages = "877--885",

journal = "Fish and Shellfish Immunology",

issn = "1050-4648",

publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - CD83 expression in sea bream macrophages is a marker for the LPS-induced inflammatory response

AU - Doñate, C.

AU - Roher, N.

AU - Balasch, J. C.

AU - Ribas, L.

AU - Goetz, F. W.

AU - Planas, J. V.

AU - Tort, L.

AU - MacKenzie, S.

PY - 2007/1/1

Y1 - 2007/1/1

N2 - CD83, a cell surface membrane glycoprotein member of the Ig superfamily which is commonly used as standard surface marker for dendritic cells, was cloned from gilthead sea bream macrophages using degenerate primers against conserved motifs of known CD83 sequences. The obtained cDNA contains an open reading frame of 669 nucleotides that translate into a 222 amino acid putative peptide. The deduced protein sequence shows conservation of features shared by vertebrate CD83 and multiple alignment with fish CD83 sequences reveals high homology. In cultured sea bream macrophages CD83 mRNA expression was significantly enhanced in a dose- and time-dependent fashion after stimulation with Escherichia coli LPS. These results indicate that in fish, macrophages express high levels of CD83 mRNA after LPS exposure and CD83 is therefore a good marker for activated mature myeloid cells in fish. © 2007 Elsevier Ltd. All rights reserved.

AB - CD83, a cell surface membrane glycoprotein member of the Ig superfamily which is commonly used as standard surface marker for dendritic cells, was cloned from gilthead sea bream macrophages using degenerate primers against conserved motifs of known CD83 sequences. The obtained cDNA contains an open reading frame of 669 nucleotides that translate into a 222 amino acid putative peptide. The deduced protein sequence shows conservation of features shared by vertebrate CD83 and multiple alignment with fish CD83 sequences reveals high homology. In cultured sea bream macrophages CD83 mRNA expression was significantly enhanced in a dose- and time-dependent fashion after stimulation with Escherichia coli LPS. These results indicate that in fish, macrophages express high levels of CD83 mRNA after LPS exposure and CD83 is therefore a good marker for activated mature myeloid cells in fish. © 2007 Elsevier Ltd. All rights reserved.

KW - CD83

KW - Fish

KW - LPS

KW - Macrophages

KW - mRNA expression

U2 - 10.1016/j.fsi.2007.03.016

DO - 10.1016/j.fsi.2007.03.016

M3 - Article

SN - 1050-4648

VL - 23

SP - 877

EP - 885

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

ER -

CD83 expression in sea bream macrophages is a marker for the LPS-induced inflammatory response

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