TY - JOUR
T1 - CD20, CD3, and CD40 ligand microclusters segregate three-dimensionally in vivo at B-cell-T-cell immunological synapses after viral immunity in primate brain
AU - Barcia, Carlos
AU - Gomez, Aurora
AU - De Pablos, Vicente
AU - Fernández-Villalba, Emiliano
AU - Liu, Chunyan
AU - Kroeger, Kurt M.
AU - Martín, Javier
AU - Barreiro, Andrés Fernández
AU - Castro, Maria G.
AU - Lowenstein, Pedro R.
AU - Herrero, Maria Trinidad
PY - 2008/10
Y1 - 2008/10
N2 - The clearance of virally infected cells from the brain is mediated by T cells that engage antigen-presenting cells to form supramolecular activation clusters at the immunological synapse. However, after clearance, the T cells persist at the infection site and remain activated locally. In the present work the long-term interactions of immune cells in brains of monkeys were imaged in situ 9 months after the viral inoculation. After viral immunity, the persistent infiltration of T cells and B cells was observed at the infection sites. T cells showed evidence of T-cell receptor signaling as a result of contacts with B cells. Three-dimensional analysis of B-cell-T-cell synapses showed clusters of CD3 in T cells and the segregation of CD20 in B cells, involving the recruitment of CD40 ligand at the interface. These results demonstrate that immunological synapses between B cells and T cells forming three-dimensional microclusters occur in vivo in the central nervous system and suggest that these interactions may be involved in the lymphocyte activation after viral immunity at the original infection site.
AB - The clearance of virally infected cells from the brain is mediated by T cells that engage antigen-presenting cells to form supramolecular activation clusters at the immunological synapse. However, after clearance, the T cells persist at the infection site and remain activated locally. In the present work the long-term interactions of immune cells in brains of monkeys were imaged in situ 9 months after the viral inoculation. After viral immunity, the persistent infiltration of T cells and B cells was observed at the infection sites. T cells showed evidence of T-cell receptor signaling as a result of contacts with B cells. Three-dimensional analysis of B-cell-T-cell synapses showed clusters of CD3 in T cells and the segregation of CD20 in B cells, involving the recruitment of CD40 ligand at the interface. These results demonstrate that immunological synapses between B cells and T cells forming three-dimensional microclusters occur in vivo in the central nervous system and suggest that these interactions may be involved in the lymphocyte activation after viral immunity at the original infection site.
UR - http://www.scopus.com/inward/record.url?scp=53749084135&partnerID=8YFLogxK
U2 - 10.1128/JVI.01326-08
DO - 10.1128/JVI.01326-08
M3 - Article
C2 - 18684835
AN - SCOPUS:53749084135
SN - 0022-538X
VL - 82
SP - 9978
EP - 9993
JO - Journal of Virology
JF - Journal of Virology
IS - 20
ER -