TY - JOUR
T1 - Bioplastic recovery from wastewater: A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures
AU - Mannina, Giorgio
AU - Presti, Dario
AU - Montiel-Jarillo, Gabriela
AU - Suárez-Ojeda, María Eugenia
N1 - Copyright © 2019 Elsevier Ltd. All rights reserved.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - © 2019 Elsevier Ltd A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures (MMCs) is proposed. PHA-accumulating capacity of the MMC was selected in a sequencing batch reactor (SBR) fed with a synthetic effluent emulating a fermented oil mill wastewater (OMW). The highest recovery yield and purity (74 ± 8% and 100 ± 5%, respectively) was obtained when using NH4-Laurate for which operating conditions of the extraction process such as temperature, concentration and contact time were optimized. Best conditions for PHA extraction from MMC turned to be: i) a pre-treatment with NaClO at 85 °C with 1 h of contact time, followed by ii) a treatment with lauric acid in a ratio acid lauric to biomass of 2:1 and 3 h of contact time.
AB - © 2019 Elsevier Ltd A new protocol for polyhydroxyalkanoates (PHA) extraction from mixed microbial cultures (MMCs) is proposed. PHA-accumulating capacity of the MMC was selected in a sequencing batch reactor (SBR) fed with a synthetic effluent emulating a fermented oil mill wastewater (OMW). The highest recovery yield and purity (74 ± 8% and 100 ± 5%, respectively) was obtained when using NH4-Laurate for which operating conditions of the extraction process such as temperature, concentration and contact time were optimized. Best conditions for PHA extraction from MMC turned to be: i) a pre-treatment with NaClO at 85 °C with 1 h of contact time, followed by ii) a treatment with lauric acid in a ratio acid lauric to biomass of 2:1 and 3 h of contact time.
KW - Bioplastic
KW - PHA
KW - Wastewater treatment
KW - Water resource recovery
KW - Bioreactors
KW - Polyhydroxyalkanoates/isolation & purification
KW - Plastics/isolation & purification
KW - Biomass
KW - Fermentation
KW - Waste Water/chemistry
KW - PERFORMANCE
KW - BIOMASS
KW - POLY(3-HYDROXYBUTYRATE) PRODUCTION
KW - ENRICHMENT
KW - EFFICIENT
KW - MICROSTRUCTURE
UR - http://www.mendeley.com/research/bioplastic-recovery-wastewater-new-protocol-polyhydroxyalkanoates-pha-extraction-mixed-microbial-cul
UR - https://www.scopus.com/pages/publications/85063012167
U2 - 10.1016/j.biortech.2019.03.037
DO - 10.1016/j.biortech.2019.03.037
M3 - Article
C2 - 30884455
SN - 0960-8524
VL - 282
SP - 361
EP - 369
JO - Bioresource Technology
JF - Bioresource Technology
ER -