Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies

Ivana Nemcakova, Lucie Blahova, Petr Rysanek, Andreu Blanquer, Lucie Bacakova, Lenka Zajíčková

Producció científica: Contribució a revistaArticleRecercaAvaluat per experts

5 Cites (Scopus)

Resum

Amine-coated biodegradable materials based on synthetic polymers have a great potential for tissue remodeling and regeneration because of their excellent processability and bioactivity. In the present study, we have investigated the influence of various chemical compositions of amine plasma polymer (PP) coatings and the influence of the substrate morphology, represented by polystyrene culture dishes and polycaprolactone nanofibers (PCL NFs), on the behavior of vascular smooth muscle cells (VSMCs). Although all amine-PP coatings improved the initial adhesion of VSMCs, 7-day long cultivation revealed a clear preference for the coating containing about 15 at.% of nitrogen (CPA-33). The CPA-33 coating demonstrated the ideal combination of good water stability, a sufficient amine group content, and favorable surface wettability and morphology. The nanostructured morphology of amine-PP-coated PCL NFs successfully slowed the proliferation rate of VSMCs, which is essential in preventing restenosis of vascular replacements in vivo. At the same time, CPA-33-coated PCL NFs supported the continuous proliferation of VSMCs during 7-day long cultivation, with no significant increase in cytokine secretion by RAW 264.7 macrophages. The CPA-33 coating deposited on biodegradable PCL NFs therefore seems to be a promising material for manufacturing small-diameter vascular grafts, which are still lacking on the current market.
Idioma originalAnglès
Nombre de pàgines24
RevistaInternational journal of molecular sciences
Volum21
Número24
DOIs
Estat de la publicacióPublicada - 12 de des. 2020

Keywords

  • Amine plasma polymer
  • Bioactive coating
  • Polycaprolactone nanofibers
  • Substrate
  • Morphology
  • Cell adhesion
  • Cell proliferation

Fingerprint

Navegar pels temes de recerca de 'Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies'. Junts formen un fingerprint únic.

Com citar-ho