Bacteria Detection at a Single-Cell Level through a Cyanotype-Based Photochemical Reaction

Jiri Dietvorst, Amparo Ferrer-Vilanova, Sharath Narayana Iyengar, Aman Russom, Núria Vigués, Jordi Mas, Lluïsa Vilaplana, Maria Pilar Marco, Gonzalo Guirado*, Xavier Muñoz-Berbel

*Autor corresponent d’aquest treball

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Resum

The detection of living organisms at very low concentrations is necessary for the early diagnosis of bacterial infections, but it is still challenging as there is a need for signal amplification. Cell culture, nucleic acid amplification, or nanostructure-based signal enhancement are the most common amplification methods, relying on long, tedious, complex, or expensive procedures. Here, we present a cyanotype-based photochemical amplification reaction enabling the detection of low bacterial concentrations up to a single-cell level. Photocatalysis is induced with visible light and requires bacterial metabolism of iron-based compounds to produce Prussian Blue. Bacterial activity is thus detected through the formation of an observable blue precipitate within 3 h of the reaction, which corresponds to the concentration of living organisms. The short time-to-result and simplicity of the reaction are expected to strongly impact the clinical diagnosis of infectious diseases.

Idioma originalEnglish
Pàgines (de-a)787-792
Nombre de pàgines6
RevistaAnalytical Chemistry
Volum94
Número2
DOIs
Estat de la publicacióPublicada - 18 de gen. 2022

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