Automatic analysis of silver-stained comets by CellProfiler software

J. E. Gonzalez; I. Romero; J. F. Barquinero; O. Garcia

doi:10.1016/j.mrgentox.2012.06.007

Automatic analysis of silver-stained comets by CellProfiler software

J. E. Gonzalez, I. Romero, J. F. Barquinero, O. Garcia

Radiobiol Lab

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The comet assay is one of the most widely used methods to evaluate DNA damage and repair in eukaryotic cells. The comets can be measured by software, in a semi-automatic or automatic process. In this paper, we apply the CellProfiler open-source software for automatic analysis of comets from digitized images, reporting the percentage of tail DNA. A side-by-side comparison of CellProfiler with CASP software demonstrated good agreement between the two packages. Our work demonstrates that automatic measurement of silver-stained comets with open-source software is possible, providing significant time savings. (c) 2012 Elsevier B.V. All rights reserved.

Idioma original	Anglès
Pàgines (de-a)	60-64
Nombre de pàgines	5
Revista	Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Volum	748
Número	1-2
DOIs	https://doi.org/10.1016/j.mrgentox.2012.06.007
Estat de la publicació	Publicada - 9 d’oct. 2012

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10.1016/j.mrgentox.2012.06.007

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title = "Automatic analysis of silver-stained comets by CellProfiler software",

abstract = "The comet assay is one of the most widely used methods to evaluate DNA damage and repair in eukaryotic cells. The comets can be measured by software, in a semi-automatic or automatic process. In this paper, we apply the CellProfiler open-source software for automatic analysis of comets from digitized images, reporting the percentage of tail DNA. A side-by-side comparison of CellProfiler with CASP software demonstrated good agreement between the two packages. Our work demonstrates that automatic measurement of silver-stained comets with open-source software is possible, providing significant time savings. (c) 2012 Elsevier B.V. All rights reserved.",

keywords = "Casp, CellProfiler, Comet assay, Silver staining",

author = "Gonzalez, {J. E.} and I. Romero and Barquinero, {J. F.} and O. Garcia",

year = "2012",

month = oct,

day = "9",

doi = "10.1016/j.mrgentox.2012.06.007",

language = "English",

volume = "748",

pages = "60--64",

journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis",

issn = "1383-5718",

publisher = "Elsevier",

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TY - JOUR

T1 - Automatic analysis of silver-stained comets by CellProfiler software

AU - Gonzalez, J. E.

AU - Romero, I.

AU - Barquinero, J. F.

AU - Garcia, O.

PY - 2012/10/9

Y1 - 2012/10/9

N2 - The comet assay is one of the most widely used methods to evaluate DNA damage and repair in eukaryotic cells. The comets can be measured by software, in a semi-automatic or automatic process. In this paper, we apply the CellProfiler open-source software for automatic analysis of comets from digitized images, reporting the percentage of tail DNA. A side-by-side comparison of CellProfiler with CASP software demonstrated good agreement between the two packages. Our work demonstrates that automatic measurement of silver-stained comets with open-source software is possible, providing significant time savings. (c) 2012 Elsevier B.V. All rights reserved.

AB - The comet assay is one of the most widely used methods to evaluate DNA damage and repair in eukaryotic cells. The comets can be measured by software, in a semi-automatic or automatic process. In this paper, we apply the CellProfiler open-source software for automatic analysis of comets from digitized images, reporting the percentage of tail DNA. A side-by-side comparison of CellProfiler with CASP software demonstrated good agreement between the two packages. Our work demonstrates that automatic measurement of silver-stained comets with open-source software is possible, providing significant time savings. (c) 2012 Elsevier B.V. All rights reserved.

KW - Casp

KW - CellProfiler

KW - Comet assay

KW - Silver staining

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DO - 10.1016/j.mrgentox.2012.06.007

M3 - Article

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JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

IS - 1-2

ER -

Automatic analysis of silver-stained comets by CellProfiler software

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