TY - JOUR
T1 - Association between the infectivity of Mycobacterium tuberculosis strains and their efficiency for extrarespiratory infection
AU - De Viedma, Darío García
AU - Lorenzo, Gema
AU - Cardona, Pere Joan
AU - Rodriguez, Noelia Alonso
AU - Gordillo, Sergi
AU - Serrano, María Jesús Ruiz
AU - Bouza, Emilio
N1 - Funding Information:
Received 23 March 2005; accepted 17 June 2005; electronically published 15 November 2005. Presented in part: 41st European Congress of Clinical Microbiology and Infectious Diseases, Glasgow, Scotland, 10–13 May 2003 (abstract P-1646). Potential conflicts of interest: none reported. Financial support: Comunidad de Madrid (grant 08.2/0029.1/2001 and GR/SAL/ 0488/2004); Fondo de Investigaciones Sanitarias (grant FIS PI020882 and research contract FIS 01/3104 to P.-J.C.); Consejería de Educación de la Comunidad de Madrid and European Social Fund (grant 7580/2003 to N.A.R.). Reprints or correspondence: Dr. Darío García de Viedma, Servicio de Microbiología y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, C/Dr Esquerdo 46, 28007 Madrid, Spain ([email protected]).
PY - 2005/12/15
Y1 - 2005/12/15
N2 - Extrarespiratory tuberculosis is determined mainly by impaired immunity of the host. The additional role played by bacterial factors in determining whether an infection by Mycobacterium tuberculosis disseminates to extrarespiratory sites has not been analyzed in depth. In the present study, we selected patients who were dually infected with 2 M. tuberculosis strains but in whom only one of the strains infected extrarespiratory sites, whereas the other strain remained at the respiratory site. We compared the infectivity of respiratory and extrarespiratory strains in a newly designed ex vivo competitive macrophage coinfection assay and in the murine aerosol-infection model. The extrarespiratory strains infected macrophages more efficiently than did the respiratory strains, and a representative extrarespiratory strain also showed higher infectivity in vivo. Our data indicate that, in addition to host immune status, a bacterial factor-the infectivity of a M. tuberculosis strain-should be considered in determining the likelihood of extrarespiratory dissemination.
AB - Extrarespiratory tuberculosis is determined mainly by impaired immunity of the host. The additional role played by bacterial factors in determining whether an infection by Mycobacterium tuberculosis disseminates to extrarespiratory sites has not been analyzed in depth. In the present study, we selected patients who were dually infected with 2 M. tuberculosis strains but in whom only one of the strains infected extrarespiratory sites, whereas the other strain remained at the respiratory site. We compared the infectivity of respiratory and extrarespiratory strains in a newly designed ex vivo competitive macrophage coinfection assay and in the murine aerosol-infection model. The extrarespiratory strains infected macrophages more efficiently than did the respiratory strains, and a representative extrarespiratory strain also showed higher infectivity in vivo. Our data indicate that, in addition to host immune status, a bacterial factor-the infectivity of a M. tuberculosis strain-should be considered in determining the likelihood of extrarespiratory dissemination.
UR - http://www.scopus.com/inward/record.url?scp=28844499578&partnerID=8YFLogxK
U2 - 10.1086/498245
DO - 10.1086/498245
M3 - Article
C2 - 16288368
AN - SCOPUS:28844499578
SN - 0022-1899
VL - 192
SP - 2059
EP - 2065
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 12
ER -