TY - JOUR
T1 - Assay of succinate dehydrogenase activity by a colorimetric-continuous method using iodonitrotetrazolium chloride as electron acceptor
AU - Munujos, Petraki
AU - Coll-Canti, Jaume
AU - Gonzalez-Sastre, Francesc
AU - Gella, F. Javier
PY - 1993/1/1
Y1 - 1993/1/1
N2 - A spectrophotometric assay method for determining succinate dehydrogenase activity is described in which iodonitrotetrazolium chloride is used as a final electron acceptor. The enzyme activity is determined by measuring the formation of formazan due to the tetrazolium salt reduction. The assay is continuous, rapid, simple, and sensitive, and may be used in the determination of enzyme activity either in tissue homogenates or as a marker of the mitochondrial fraction in cell fractionation procedures. © 1994 Academic Press, Inc. All rights reserved.
AB - A spectrophotometric assay method for determining succinate dehydrogenase activity is described in which iodonitrotetrazolium chloride is used as a final electron acceptor. The enzyme activity is determined by measuring the formation of formazan due to the tetrazolium salt reduction. The assay is continuous, rapid, simple, and sensitive, and may be used in the determination of enzyme activity either in tissue homogenates or as a marker of the mitochondrial fraction in cell fractionation procedures. © 1994 Academic Press, Inc. All rights reserved.
U2 - 10.1006/abio.1993.1360
DO - 10.1006/abio.1993.1360
M3 - Article
SN - 0003-2697
VL - 212
SP - 506
EP - 509
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -