TY - JOUR
T1 - Aptamers based electrochemical biosensor for protein detection using carbon nanotubes platforms
AU - Kara, Pinar
AU - de la Escosura-Muñiz, Alfredo
AU - Maltez-da Costa, Marisa
AU - Guix, Maria
AU - Ozsoz, Mehmet
AU - Merkoçi, Arben
PY - 2010/12/15
Y1 - 2010/12/15
N2 - A label-free bioelectronic detection of aptamer-thrombin interaction based on electrochemical impedance spectroscopy (EIS) technique is reported. Multiwalled carbon nanotubes (MWCNTs) were used as modifiers of screen-printed carbon electrotransducers (SPCEs), showing improved characteristics compared to the bare SPCEs. 5'amino linked aptamer sequence was immobilized onto the modified SPCEs and then the binding of thrombin to aptamer sequence was monitored by EIS transduction of the resistance to charge transfer (Rct) in the presence of 5mM [Fe(CN)6]3-/4-, obtaining a detection limit of 105pM. This study represents an alternative electrochemical biosensor for the detection of proteins with interest for future applications. © 2010 Elsevier B.V.
AB - A label-free bioelectronic detection of aptamer-thrombin interaction based on electrochemical impedance spectroscopy (EIS) technique is reported. Multiwalled carbon nanotubes (MWCNTs) were used as modifiers of screen-printed carbon electrotransducers (SPCEs), showing improved characteristics compared to the bare SPCEs. 5'amino linked aptamer sequence was immobilized onto the modified SPCEs and then the binding of thrombin to aptamer sequence was monitored by EIS transduction of the resistance to charge transfer (Rct) in the presence of 5mM [Fe(CN)6]3-/4-, obtaining a detection limit of 105pM. This study represents an alternative electrochemical biosensor for the detection of proteins with interest for future applications. © 2010 Elsevier B.V.
KW - Aptamer
KW - Aptasensor
KW - Carbon nanotubes
KW - Electrochemical impedance spectroscopy
KW - Screen-printed electrodes
KW - Thrombin
U2 - 10.1016/j.bios.2010.07.090
DO - 10.1016/j.bios.2010.07.090
M3 - Article
SN - 0956-5663
VL - 26
SP - 1715
EP - 1718
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 4
ER -