Aptamers based electrochemical biosensor for protein detection using carbon nanotubes platforms

Pinar Kara, Alfredo de la Escosura-Muñiz, Marisa Maltez-da Costa, Maria Guix, Mehmet Ozsoz, Arben Merkoçi

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Resum

A label-free bioelectronic detection of aptamer-thrombin interaction based on electrochemical impedance spectroscopy (EIS) technique is reported. Multiwalled carbon nanotubes (MWCNTs) were used as modifiers of screen-printed carbon electrotransducers (SPCEs), showing improved characteristics compared to the bare SPCEs. 5'amino linked aptamer sequence was immobilized onto the modified SPCEs and then the binding of thrombin to aptamer sequence was monitored by EIS transduction of the resistance to charge transfer (Rct) in the presence of 5mM [Fe(CN)6]3-/4-, obtaining a detection limit of 105pM. This study represents an alternative electrochemical biosensor for the detection of proteins with interest for future applications. © 2010 Elsevier B.V.
Idioma originalAnglès
Pàgines (de-a)1715-1718
RevistaBiosensors and Bioelectronics
Volum26
Número4
DOIs
Estat de la publicacióPublicada - 15 de des. 2010

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