TY - JOUR
T1 - Application of commercial and non-commercial immobilized lipases for biocatalytic production of ethyl lactate in organic solvents
AU - Koutinas, Michalis
AU - Yiangou, Chrystalleni
AU - Osório, Natália M.
AU - Ioannou, Katerina
AU - Canet, Albert
AU - Valero, Francisco
AU - Ferreira-Dias, Suzana
PY - 2018/1/1
Y1 - 2018/1/1
N2 - © 2017 This study explores the potential for enhancing the production of ethyl lactate (EL), a green solvent, through enzymatic esterification. Different solvents were compared as organic media for conversion of lactate and ethanol into EL, catalyzed by Novozym 435. Chloroform and hexane were the most effective in low acid concentrations (0.01–0.1 M) exhibiting maximum EL yields of 88% and 75% respectively. The yield of EL improved as the solvent's Log P increased up to a value of 2. Non-commercial immobilized biocatalysts consisting heterologous Rhizopous oryzae (rROL) and Candida rugosa (CRL) lipases immobilized on hydrophobic supports were compared to commercial biocatalysts clarifying that Novozym 435 and Lipozyme RM IM could be efficiently applied. Operational stability tests were conducted using Novozym 435, which retained higher activity in chloroform as compared to hexane. Although non-commercial biocatalysts were not competitive in esterification, they exhibited significant activity towards hydrolysis constituting a valuable alternative to higher-cost options.
AB - © 2017 This study explores the potential for enhancing the production of ethyl lactate (EL), a green solvent, through enzymatic esterification. Different solvents were compared as organic media for conversion of lactate and ethanol into EL, catalyzed by Novozym 435. Chloroform and hexane were the most effective in low acid concentrations (0.01–0.1 M) exhibiting maximum EL yields of 88% and 75% respectively. The yield of EL improved as the solvent's Log P increased up to a value of 2. Non-commercial immobilized biocatalysts consisting heterologous Rhizopous oryzae (rROL) and Candida rugosa (CRL) lipases immobilized on hydrophobic supports were compared to commercial biocatalysts clarifying that Novozym 435 and Lipozyme RM IM could be efficiently applied. Operational stability tests were conducted using Novozym 435, which retained higher activity in chloroform as compared to hexane. Although non-commercial biocatalysts were not competitive in esterification, they exhibited significant activity towards hydrolysis constituting a valuable alternative to higher-cost options.
KW - Esterification
KW - Ethyl lactate
KW - Lipase
KW - Novozym 435
KW - Operational stability
U2 - 10.1016/j.biortech.2017.09.130
DO - 10.1016/j.biortech.2017.09.130
M3 - Article
C2 - 28968571
SN - 0960-8524
VL - 247
SP - 496
EP - 503
JO - Bioresource Technology
JF - Bioresource Technology
ER -