TY - JOUR
T1 - Application of a validated high-performance liquid chromatography-mass spectrometry assay to the analysis of m- and p-hydroxybenzoylecgonine in meconium
AU - Pichini, S.
AU - Marchei, E.
AU - Pacifici, R.
AU - Pellegrini, M.
AU - Lozano, Jaime
AU - García-Algar, O.
PY - 2005/6/5
Y1 - 2005/6/5
N2 - A high-performance liquid chromatography (HPLC)-mass spectrometry (MS) assay, already validated for opiates and cocaine in meconium, has been re-applied for determination of m- and p-hydroxybenzoylecgonine, using nalorphine as the internal standard. Methodology included an initial extraction from the matrix by methanol and then a solid-phase extraction (SPE). A reversed-phase chromatography was used with a gradient of 1% acetic acid-acetonitrile coupled to atmospheric pressure ionization electrospray-mass spectrometry single ion monitoring mode. This method, validated in the range 0.005-1.00 μg analytes/g meconium, proved useful to identify and quantify these two metabolites in meconium samples, already tested for the presence of cocaine, benzoylecgonine and cocaethylene. A positivity of range of concentrations varied between 0.007 and 0.338 μg/g, confirming the importance of these two hydroxylated derivatives to monitor fetal exposure to cocaine. © 2005 Elsevier B.V. All rights reserved.
AB - A high-performance liquid chromatography (HPLC)-mass spectrometry (MS) assay, already validated for opiates and cocaine in meconium, has been re-applied for determination of m- and p-hydroxybenzoylecgonine, using nalorphine as the internal standard. Methodology included an initial extraction from the matrix by methanol and then a solid-phase extraction (SPE). A reversed-phase chromatography was used with a gradient of 1% acetic acid-acetonitrile coupled to atmospheric pressure ionization electrospray-mass spectrometry single ion monitoring mode. This method, validated in the range 0.005-1.00 μg analytes/g meconium, proved useful to identify and quantify these two metabolites in meconium samples, already tested for the presence of cocaine, benzoylecgonine and cocaethylene. A positivity of range of concentrations varied between 0.007 and 0.338 μg/g, confirming the importance of these two hydroxylated derivatives to monitor fetal exposure to cocaine. © 2005 Elsevier B.V. All rights reserved.
KW - LC-MS
KW - m-Hydroxybenzoylecgonine
KW - Meconium
KW - p-Hydroxybenzoylecgonine
U2 - 10.1016/j.jchromb.2005.03.027
DO - 10.1016/j.jchromb.2005.03.027
M3 - Article
SN - 1570-0232
VL - 820
SP - 151
EP - 156
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 1
ER -