TY - CHAP
T1 - Antigen discovery in bacterial panproteomes
AU - Yero, Daniel
AU - Conchillo-Solé, Oscar
AU - Daura, Xavier
PY - 2020/9/22
Y1 - 2020/9/22
N2 - There is still a lack of vaccines for many bacterial infections for which the best treatment option would be a prophylactic one. On the other hand, effectiveness has been questioned for some existing vaccines, prompting new developments. Therapeutic vaccines are also becoming a treatment option in specific cases where antibiotics tend to fail. In this scenario, refinement and extension of the classical reverse vaccinology approach is allowing scientists to find new and more effective antigens. In this chapter, we describe an in silico methodology that integrates pangenomic, immunoinformatic, structural, and evolutionary approaches for the screening of potential antigens in a given bacterial species. The strategy focuses on targeting relatively conserved epitopes in core proteins to design broadly cross-protective vaccines and avoid allele-specific immunity. The proposed methodological steps and computational tools can be easily implemented in a reverse vaccinology approach not only to identify new leads with strong immune response but also to develop diagnostic assays.
AB - There is still a lack of vaccines for many bacterial infections for which the best treatment option would be a prophylactic one. On the other hand, effectiveness has been questioned for some existing vaccines, prompting new developments. Therapeutic vaccines are also becoming a treatment option in specific cases where antibiotics tend to fail. In this scenario, refinement and extension of the classical reverse vaccinology approach is allowing scientists to find new and more effective antigens. In this chapter, we describe an in silico methodology that integrates pangenomic, immunoinformatic, structural, and evolutionary approaches for the screening of potential antigens in a given bacterial species. The strategy focuses on targeting relatively conserved epitopes in core proteins to design broadly cross-protective vaccines and avoid allele-specific immunity. The proposed methodological steps and computational tools can be easily implemented in a reverse vaccinology approach not only to identify new leads with strong immune response but also to develop diagnostic assays.
KW - Bacteria
KW - Computational antigen discovery
KW - Immunoinformatics
KW - Vaccines
KW - Bacteria
KW - Computational antigen discovery
KW - Immunoinformatics
KW - Vaccines
UR - http://www.scopus.com/inward/record.url?scp=85091471506&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/e89803ec-caea-36b7-91ff-092cad0d3f9e/
U2 - 10.1007/978-1-0716-0795-4_5
DO - 10.1007/978-1-0716-0795-4_5
M3 - Capítulo
C2 - 32959240
AN - SCOPUS:85091471506
VL - 2183
T3 - Methods in Molecular Biology
SP - 43
EP - 62
BT - Methods in Molecular Biology
ER -