TY - JOUR
T1 - An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection
AU - Mayorga-Martinez, Carmen C.
AU - Chamorro-García, Alejandro
AU - Serrano, Lorena
AU - Rivas, Lourdes
AU - Quesada-Gonzalez, Daniel
AU - Altet, Laura
AU - Francino, Olga
AU - Sánchez, Armand
AU - Merkoçi, Arben
PY - 2015/7/14
Y1 - 2015/7/14
N2 - © The Royal Society of Chemistry. An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO2 NP). An aminated oligonucleotide probe is immobilized on the IrO2 NP/polythionine modified electrode and ethanolamine was used as a blocking agent. Different diluted PCR amplified DNA samples have been detected. The selectivity and reproducibility of this system are studied and the system was highly reproducible with RSD ≈ 15% and sensitive enough while using 2% of ethanolamine during the blocking step employed for genosensor preparation.
AB - © The Royal Society of Chemistry. An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO2 NP). An aminated oligonucleotide probe is immobilized on the IrO2 NP/polythionine modified electrode and ethanolamine was used as a blocking agent. Different diluted PCR amplified DNA samples have been detected. The selectivity and reproducibility of this system are studied and the system was highly reproducible with RSD ≈ 15% and sensitive enough while using 2% of ethanolamine during the blocking step employed for genosensor preparation.
U2 - 10.1039/c5tb00545k
DO - 10.1039/c5tb00545k
M3 - Article
SN - 2050-7518
VL - 3
SP - 5166
EP - 5171
JO - Journal of Materials Chemistry B
JF - Journal of Materials Chemistry B
ER -