Ability of the exopolymer excreted by Pseudoalteromonas antarctica NF3, to coat liposomes and to protect these structures against octyl glucoside)

A. De La Maza, L. Codech, O. Lopez, J. L. Parra, M. Sabes, J. Guinea

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Resum

The ability of an exopolymer of glycoproteic character (GP) excreted by a new gram-negative specie Pseudoalteromonas antarctica NF3, to coat phosphatidylcholine (PC) liposomes and to protect these bilayers against the action of the nonionic surfactant octyl glucoside (OG) has been investigated. TEM micrographs of freeze-fractured liposome/GP aggregates reveal that the addition of GP to liposomes led to the formation of a covering structure (polymer adsorbed onto the bilayers) that tightly coated PC bilayers. The complete coating was already achieved when the proportion of GP assembled with liposomes was approximately 10% (wt% vs total PC). Higher GP amounts resulted in a growth of this coating structure which exhibited at the highest GP proportion in the system (31 % of assembled GP) a multilayered structure. An increasing resistance of PC liposomes to be affected by OG both at sublytic and lytic levels occurred as the proportion of GP in the system rose; this protective effect being more effective when the proportion of assembled GP was 10-20% in weight. Thus, although a direct dependence was found between the growth of the enveloping structure and the resistance of the coated liposomes to be affected by OG, the best protection occurred when the proportion of assembled GP was about 10 wt%. © VSP 1999.
Idioma originalEnglish
Pàgines (de-a)557-572
RevistaJournal of Biomaterials Science, Polymer Edition
Volum10
DOIs
Estat de la publicacióPublicada - 1 de gen. 1999

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