TY - JOUR
T1 - A versatile perifusion system for the NMR spectroscopy of bovine retina. Assignment of resonances and effect of ischemia
AU - Cabanas, Miquel E.
AU - Alonso, Juli
AU - Arus, Carles
AU - Anderson, Mark E.
AU - Westler, William M.
AU - Markley, John L.
PY - 1993/1/1
Y1 - 1993/1/1
N2 - A perifusion system has been designed for metabolic nuclear magnetic resonance (NMR) spectroscopy studies of the bovine retina. This system, which allows the use of unmodified commercial probes for multinuclear studies, is shown here to maintain the perifused retina in a metabolic steady state at 25°C, as deduced from its 31P and 1H NMR spectra. The 31P NMR ex vivo spectrum of the retina has been characterized, and its major resonances have been assigned in tissue extracts. We have found that excised retinal tissue subjected to 2 hr of ischemia is capable, upon perifusion with oxygenated medium, of recovering its resting spectral pattern. Thus, this system offers a versatile model for studies of physiological events related with ischemia in a neural tissue, without associated systemic effects. © 1993 Academic Press, Inc.
AB - A perifusion system has been designed for metabolic nuclear magnetic resonance (NMR) spectroscopy studies of the bovine retina. This system, which allows the use of unmodified commercial probes for multinuclear studies, is shown here to maintain the perifused retina in a metabolic steady state at 25°C, as deduced from its 31P and 1H NMR spectra. The 31P NMR ex vivo spectrum of the retina has been characterized, and its major resonances have been assigned in tissue extracts. We have found that excised retinal tissue subjected to 2 hr of ischemia is capable, upon perifusion with oxygenated medium, of recovering its resting spectral pattern. Thus, this system offers a versatile model for studies of physiological events related with ischemia in a neural tissue, without associated systemic effects. © 1993 Academic Press, Inc.
KW - Bovine retina
KW - Ischemia
KW - Metabolism
KW - Nuclear magnetic resonance spectroscopy
KW - Perifusion system
U2 - 10.1006/exer.1993.1175
DO - 10.1006/exer.1993.1175
M3 - Article
SN - 0014-4835
VL - 57
SP - 669
EP - 678
JO - Experimental Eye Research
JF - Experimental Eye Research
IS - 6
ER -