A third-generation hydrogen peroxide biosensor based on horseradish peroxidase covalently immobilized on electrografted organic film on screen-printed carbon electrode

Abd Elgawad Radi, Xavier Muñoz-Berbel, Montserrat Cortina-Puig, Jean Louis Marty

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    Resum

    A new convenient strategy to fabricate a third-generation hydrogen peroxide biosensor was described. The screen-printed carbon electrode (SPCE) was first modified with a layer of 4-nitrophenyl assembled from the 4-nitroaniline diazonium salt synthesized in situ in acidic aqueous solution. Next, the nitro groups were converted to amines followed by crosslinking to the horseradish peroxidase (HRP) by glutaraldehyde. The redox chemistry of the active center of the HRP was observed and the HRP-modified electrode displayed electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2) without any mediators. H2O2 was determined in a linear range from 5.0 μM to 50.0 μM, with a detection limit of 1.0 μM. Furthermore, the biosensor exhibited fast amperometric response, good reproducibility and long-term stability. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
    Idioma originalAnglès
    Pàgines (de-a)1624-1629
    RevistaElectroanalysis
    Volum21
    Número14
    DOIs
    Estat de la publicacióPublicada - 1 de jul. 2009

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