A novel embryo identification system by direct tagging of mouse embryos using silicon-based barcodes

Sergi Novo, Leonardo Barrios, Josep Santaló, Rodrigo Gmez-Martnez, Marta Duch, Jaume Esteve, Jos Antonio Plaza, Carme Nogués, Elena Ibáñez*

*Autor corresponent d’aquest treball

Producció científica: Contribució a una revistaArticleRecercaAvaluat per experts

25 Cites (Scopus)

Resum

Background Measures to prevent assisted reproductive technologies (ART) mix-ups, such as labeling of all labware and double-witnessing protocols, are currently in place in fertility clinics worldwide. Technological solutions for electronic witnessing are also being developed. However, none of these solutions eliminate the risk of identification errors, because gametes and embryos must be transferred between containers several times during an ART cycle. Thus, the objective of this study was to provide a proof of concept for a direct embryo labeling system using silicon-based barcodes. Methods Three different types of silicon-based barcodes (A, B and C) were designed and manufactured, and microinjected into the perivitelline space of mouse pronuclear embryos (one to four barcodes per embryo). Embryos were cultured in vitro until the blastocyst stage, and rates of embryo development, retention of the barcodes in the perivitelline space and embryo identification were assessed every 24 h. Release of the barcodes after embryo hatching was also determined. Finally, embryos microinjected with barcodes were frozen and thawed at the 2-cell stage to test the validity of the system after cryopreservation. Results Barcodes present in the perivitelline space, independently of their type and number, did not affect embryo development rates. The majority of embryos (>90) retained at least one of the microinjected barcodes in their perivitelline space up to the blastocyst stage. Increasing the number of barcodes per embryo resulted in a significant increase in embryo identification rates, but a significant decrease in the barcode release rates after embryo hatching. The highest rates of successful embryo identification (97) were achieved with the microinjection of four type C barcodes, and were not affected by cryopreservation. Conclusions Our results demonstrate the feasibility of a direct embryo labeling system and constitute the starting point in the development of such systems. © 2010 The Author.
Idioma originalEnglish
Pàgines (de-a)96-105
RevistaHuman Reproduction
Volum26
DOIs
Estat de la publicacióPublicada - 1 de gen. 2011

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