A Novel 3D-Printed/Porous Conduit with Tunable Properties to Enhance Nerve Regeneration Over the Limiting Gap Length

Eugenio Redolfi-Riva, Míriam Pérez-Izquierdo, Ciro Zinno, Estefania Contreras, Bruno Rodríguez-Meana, Federica Iberite, Leonardo Ricotti, Silvestro Micera*, Xavier Navarro*

*Autor corresponent d’aquest treball

Producció científica: Contribució a una revistaArticleRecercaAvaluat per experts

2 Cites (Scopus)

Resum

Engineered grafts constitute an alternative to autologous transplant for repairing severe peripheral nerve injuries. However, current clinically available solutions have substantial limitations and are not suited for the repair of long nerve defects. A novel design of nerve conduit is presented here, which consists of a chitosan porous matrix embedding a 3D-printed poly-ε-caprolactone mesh. These materials are selected due to their high biocompatibility, safe degradability, and ability to support the nerve regeneration process. The proposed design allows high control over geometrical features, pores morphology, compression resistance, and bending stiffness, yielding tunable and easy-to-manipulate grafts. The conduits are tested in chronic animal experiments, aiming to repair a 15-mm long gap in the sciatic nerve of rats, and the results are compared with an autograft. Electrophysiological and nociception tests performed monthly during a 4-month follow-up show that these conduits allow a good degree of muscle functional recovery. Histological analyses show abundant cellularization in the wall and in the lumen of the conduits and regenerated axons within all rats treated with these grafts. It is suggested that the proposed conduits have the potential to repair nerves over the limiting gap length and can be proposed as strategy to overcome the limitations of autograft.
Idioma originalEnglish
Número d’article2300136
Nombre de pàgines19
RevistaAdvanced Materials Technologies
Volum8
Número17
DOIs
Estat de la publicacióPublicada - 11 de set. 2023

Keywords

  • chitosan
  • Limiting gap length
  • Nerve conduits
  • Nerve injury
  • Nerve regeneration
  • PLC

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