A Dbf4 mutant contributes to bypassing the Rad53-mediated block of origins of replication in response to genotoxic stress.

Alba Duch, Gloria Palou, Zophonias O. Jonsson, Roger Palou, Enrique Calvo, James Wohlschlegel, David G. Quintana

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Resum

An intra-S phase checkpoint slows the rate of DNA replication in response to DNA damage and replication fork blocks in eukaryotic cells. In the budding yeast Saccharomyces cerevisiae, such down-regulation is achieved through the Rad53 kinase-dependent block of origins of replication. We have identified the Rad53 phosphorylation sites on Dbf4, the activator subunit of the essential S phase Dbf4-dependent kinase, and generated a non-phosphorylatable Dbf4 mutant (dbf4(7A)). We show here that dbf4(7A) is a bona fide intra-S phase checkpoint bypass allele that contributes to abrogating the Rad53 block of origin firing in response to genotoxic stress.

Idioma originalEnglish
Pàgines (de-a)2486-2491
Nombre de pàgines6
RevistaJournal of Biological Chemistry
Volum286
Número4
DOIs
Estat de la publicacióPublicada - 28 de gen. 2011

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