Replication Data for: Polylactic acid nanoplastics (PLA-NPLs) induce adverse effects on an in vitro model of the human lung epithelium: the Calu-3 air-liquid interface (ALI) barrier

This dataset includes the images published in the paper "Polylactic Acid Nanoplastics (PLA-NPLs) Induce Adverse Effects on an In Vitro Model of the Human Lung Epithelium: The Calu-3 Air-Liquid Interface (ALI) Barrier." <br> Specifically, it contains:<br> <ul><li>TEM images characterizing the Calu-3 barrier establishment. Air liquid Interface lung epithelial images specifying the different observed characteristics.</li> <li>A SEM image of the PLA nanoparticles used in the study (PH_PLA).</li> <li>Confocal images illustrating particle internalisation at different time points. 3D images of Calu-3 barriers untreated and treated to PLA-NPLs for 24 h and 2 weeks, respectively. Orthogonal views demonstrating the presence of PLA-NPLs inside the cell cytoplasm. 3D images with cross-sectional views demonstrating the presence of PLA-NPLs in the nuclear compartment. PLA-NPLs, cell nuclei, and ZO-1 junctions are seen in green, blue, and red, respectively. </li> <li>Confocal images of immunostaining of tight junctions after PLA treatment. Images of untreated, and 24 hours and 2 weeks treated Calu-3 barriers with 2.5 µg/cm2 PLA-NPLs. In blue the cell nuclei and in red the tight junction ZO-1.</li> <li>Inverted light microscopy images of Calu-3 barriers stained with Alcian Blue and hematoxylin-eosin, highlighting mucus, cellular nuclei, and cytoplasm.</li></ul>