Replication data for "A rewired NADPH-dependent redox shuttle for testing peroxisomal compartmentalization of synthetic metabolic pathways in Komagataella phaffii"

Supplementary files of research article "A rewired NADPH-dependent redox shuttle for testing peroxisomal compartmentalization of synthetic metabolic pathways in Komagataella phaffii". This study describes the establishment of the synthetic malonyl-CoA pathway in the peroxisome of Komagataella phaffii for 3-HP production using renewable carbon sources. Several strains were constructed and tested on 24-deep-well plate cultivations containing Buffered Minimal medium supplemented with the appropriate carbon source: glucose (BMD), glycerol (BMG), methanol (BMM), and oleic acid (BMO). Three independent transformants for each strain were cultivated.